文献类型：期刊文献

英文题名：Lactiplantibacillus Plantarum GUANKE Alleviates Allergic Asthma by Restricting Monocyte-to-DC Differentiation Via Microbiota-Associated IAA

作者：He, Yujia[1,2];Mi, Jielan[1,2,3];Zhao, Zhiping[4];Zhang, Xiao[1,2,5];Yang, Zhihan[1,2];Yue, Kun[1,2,6];Huang, Yuanming[1,2];Song, Liqiong[1,2];Wen, Xiaoting[4];Xu, Jianguo[1,2];Ren, Zhihong[1,2]

第一作者：He, Yujia

通信作者：Xu, JG[1];Ren, ZH[1];Xu, JG[2];Ren, ZH[2];Wen, XT[3]

机构：[1]Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Natl Key Lab Intelligent Tracking & Forecasting In, Beijing, Peoples R China;[2]Chinese Acad Prevent Med, Beijing, Peoples R China;[3]Nankai Univ, Sch Med, Inst Publ Hlth, Tianjin, Peoples R China;[4]Shanxi Med Univ, Shanxi Acad Med Sci, Shanxi Bethune Hosp, Hosp 3, Taiyuan, Peoples R China;[5]Gansu Univ Chinese Med, Lanzhou, Gansu, Peoples R China;[6]Chinese Acad Med Sci & Peking Union Med Coll, Chinese Acad Med Sci, Inst Pathogen Biol, Res Unit Unknown Microbe, Beijing, Peoples R China

第一机构：Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Natl Key Lab Intelligent Tracking & Forecasting In, Beijing, Peoples R China

通信机构：[1]corresponding author), Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Natl Key Lab Intelligent Tracking & Forecasting In, Beijing, Peoples R China;[2]corresponding author), Chinese Acad Prevent Med, Beijing, Peoples R China;[3]corresponding author), Shanxi Med Univ, Shanxi Acad Med Sci, Shanxi Bethune Hosp, Hosp 3, Taiyuan, Peoples R China.

年份：2026

外文期刊名：ADVANCED SCIENCE

收录：;EI(收录号：20263821508583);Scopus(收录号：2-s2.0-105050692356);WOS:【SCI-EXPANDED(收录号:WOS:001876813000001)】；

基金：This work was supported by grants from Detection Technology Reserve for Highly Pathogenic Pathogens Posing Biosafety Threats (Grant Nos. 2026NITFID512, 01058652100409004) and the National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention (Grant No. 33054 and 29172), the National Natural Science Foundation of China (Grant No. 82202005).

语种：英文

外文关键词：asthma; CX3CR1; dendritic cell; indole-3-acetic acid; NOTCH4

摘要：Emerging evidence highlights the role of the gut microbiota in allergic asthma, with microbial metabolites mediating distal immune responses. This study investigated the protective efficacy of Lactiplantibacillus plantarum GUANKE and the mechanisms associated with dendritic cell (DC)-driven allergic airway inflammation (AAI). GUANKE conferred both prophylactic and therapeutic protection by attenuating airway hyperresponsiveness and Th2-driven inflammation while partially restoring regulatory T cell (Treg) population. Antibiotic depletion and fecal microbiota transfer indicated that GUANKE-mediated protection was associated with remodeling of the indigenous microbiota and microbiota-dependent restoration of circulating indole-3-acetic acid (IAA). Targeted serum metabolomics further revealed lower circulating IAA levels in patients with asthma than in healthy controls. Exogenous IAA reduced CX3CR1+Ly6C- monocytic precursors and pulmonary CD11b+ DC accumulation. These reductions were preserved after CD11c-specific aryl hydrocarbon receptor (AhR) deletion and accompanied by reduced JAK3-STAT3 signaling, lower STAT3 occupancy at the Jag1 promoter, and attenuation of the Jag1-Notch4-Hey1 axis. Pharmacological STAT3 activation or Jagged1-mediated Notch reactivation partially reversed the effects of IAA. Collectively, these findings link microbiota-associated IAA restoration to GUANKE-mediated protection and implicate STAT3-Jag1-Notch4 signaling in monocyte-to-DC differentiation during AAI.