文献类型：期刊文献

中文题名：地黄饮子孵育骨髓间充质干细胞对大鼠缺血性脑损伤Bcl-2及Bax表达的影响

英文题名：Effect of Dihuang Yinzi incubated bone marrow stromal stem cells on expression of Bcl-2 and Bax in cerebral ischemic damage in rats

作者：程小丽[1];刘永琦[1];潘政[2];吴晓晶[1];范萍[1];窦娟娟[1];董介正[1];颜春鲁[1]

第一作者：程小丽

机构：[1]甘肃中医学院基础课部;[2]兰州大学第二医院

第一机构：甘肃中医药大学

年份：2011

卷号：26

期号：1

起止页码：153

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：国家中医药管理局基金项目(No.04-05JQ05);甘肃省自然基金项目(No.3ZS051-A)~~

语种：中文

中文关键词：缺血性脑损伤;骨髓间充质干细胞;地黄饮子;B淋巴细胞性白血病-2;Bcl-2相关X蛋白

外文关键词：Ischemic brain damage; Bone marrow stromal stem cells; Dihuang Yinzi; Bcl-2; Bax

摘要：目的:探讨地黄饮子孵育骨髓间充质干细胞(BMSCs)移植对鼠缺血性脑损伤的脑组织Bcl-2(B淋巴细胞性白血病-2)及Bax(Bcl-2相关X蛋白)表达的影响作用。方法:选用健康SPF级Wistar大鼠随机分为假手术组、模型组、BMSCs组、维甲酸组和地黄饮子组。用线栓法制成大鼠大脑中动脉缺血(MCAO)模型,术后24h,假手术、模型组大鼠尾静脉注射生理盐水,其余各组大鼠经尾静脉输入等量相应药物诱导后的BMSCs。移植7d和14d后断头取脑,用免疫组化法染色,分别观察大脑皮质每高倍镜视野Bcl-2、Bax的阳性表达情况。结果:与假手术组比较,模型组缺血性脑损伤时大脑皮层均有Bcl-2和Bax的表达明显增加(P<0.05);地黄饮子组、维甲酸组、BMSCs组与假手术组、模型组比较,缺血性脑损伤时皮层Bcl-2的表达均显著增高(P<0.05),Bax的表达均明显降低(P<0.05)。结论:Bcl-2和Bax在缺血性脑损伤过程中发挥重要作用,地黄饮子对缺血性脑损伤具有保护作用,其保护作用机制可能与提高Bcl-2表达、抑制Bax表达而减少细胞凋亡有关。
Objective： To observe the effect of Dihuang Yinzi incubate bone marrow stromal stem cells on the expressions of Bcl-2 and Bax in cerebral ischemic damage in Rats.Methods：The healthy Wistar rats were divided into the sham-operated group,model group,BMSCs group,RA group and Dihuang Yinzi group.Permanent models of middle cerebral artery occlusion by nylon suture embolization were made in rats.After operated 24h,the sham-operated group and model group were injected isotonic Na chloride through vena caudalis,the other groups were injected medicine to lead BMSCs.After 7 days and 14 days the rats were killed then the brains were taken out.Immunohistochemical methed was used to investigate the expressions of Bcl-2 and Bax in the cerebral cortex.Results： Compared with the sham-operated group,the expressions of Bcl-2 and Bax increased in cerebral ischemia in model group（P0.05）.Compared with the sham-operated group and model group,the expressions of Bcl-2 in BMSCs group,RA group and Di Huang Yin Zi group increased（P0.05）.However,the expressions of Bax decreased（P0.05）.Conclusion： Bcl-2 and Bax have the important effect in the procedure of cerebral ischemic injury.Dihuang Yinzi has protection for the cerebral ischemic injury,which maybe related to increase the expressions of bcl-2 and decreasethe expressions of bax.