文献类型：期刊文献

英文题名：Differential Expression of Serum Proteins in Rats with Allergic Asthma: A Study Based on the Nanoliter Two-Dimensional Liquid Chromatography Technique

作者：Yan, Xingke[1];Zhang, Ao[1];Yu, Lu[2];Chen, Cheng[3];Cui, Haifu[4]

第一作者：严兴科

通信作者：Zhang, A[1]

机构：[1]Gansu Univ Chinese Med, Coll Acupuncture Moxibust & Tuina, Lanzhou 730000, Peoples R China;[2]Jilin Prov Jilin Hosp Integrated Tradit Chinese &, Jilin 132021, Jilin, Peoples R China;[3]Daqing Chinese Med Hosp, Daqing 163311, Peoples R China;[4]Hunchun Chinese Med Hosp, Hunchun 133300, Peoples R China

第一机构：甘肃中医药大学针灸推拿学院

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Acupuncture Moxibust & Tuina, Lanzhou 730000, Peoples R China.|[10735db5df93766a96e5b]甘肃中医药大学针灸推拿学院;[10735]甘肃中医药大学;

年份：2017

卷号：2017

起止页码：1

外文期刊名：CANADIAN RESPIRATORY JOURNAL

收录：;Scopus(收录号：2-s2.0-85018845287);WOS:【SCI-EXPANDED(收录号:WOS:000399924200001)】；

基金：This study was supported by Henan Shao's Acupuncture School Inheritance Studio & Gansu University of Chinese Medicine Workstation Project of State Administration of Traditional Chinese Medicine (no. LP0116036-z8), the Basic Research Foundation of Higher Education Institutions of Gansu Provincial Department of Finance (no. 2011-4), and the Introduction of Talent Scientific Research Start-Up Foundation of Gansu University of Chinese Medicine (no. 2012-7).

语种：英文

摘要：Objective. To investigate the pathogenesis of allergic asthma via the nanoliter two-dimensional liquid chromatography (nano-2D-LC) technique. Method. 24 Wistar rats were randomized into 3 groups: the blank-control group (A), the restrained group (B), and the asthma model group (C). The nanoliter two-dimensional liquid chromatography (nano-2D-LC) technique was used to study the differential protein expressions of the serum in asthmatic rats. Results. Compared with the restrained group, the data from the asthma model group displayed a distinctive peak, that is, peak number 13 (94.731 min). The data also displayed three missing peaks in the asthma group, that is, peak number 1 (77.489 min), peak number 2 (78.418 min), and peak number 5 (80.533 min), suggesting that the corresponding peptides might be related to the pathogenesis of asthma. Compared with the blank-control group, the restrained group showed 4 new peaks, that is, peak number 2 (78.418 min), peak number 4 (79.398 min), peak number 5 (80.533 min), and peak number 7 (81.824 min). The restrained group also displayed a missing peak, that is, peak number 3 (78.804 min), indicating that those 5 polypeptides might be related to the binding-induced stress stimuli. Conclusion. The study suggests that the pathogenesis of allergic asthma is closely related to abnormal levels of proteins; however, future animal experiments should identify the specific protein expressions caused by stress factors.