文献类型：期刊文献

英文题名：A novel SARS-CoV-2 related coronavirus with complex recombination isolated from bats in Yunnan province, China

作者：Li, Li-li[1,2];Wang, Jing-lin[3];Ma, Xiao-hua[1];Sun, Xiao-man[1,2,4];Li, Jin-song[1,2];Yang, Xiao-fei[5];Shi, Wei-feng[6];Duan, Zhao-jun[1,2]

第一作者：Li, Li-li

通信作者：Duan, ZJ[1]

机构：[1]China CDC, Natl Inst Viral Dis Control & Prevent, 100 Ying Xin St, Beijing 100052, Peoples R China;[2]Natl Hlth Commiss Peoples Republ China, Key Lab Med Virol & Viral Dis, Beijing, Peoples R China;[3]Yunnan Anim Sci & Vet Inst, Yunnan Trop & Subtrop Anim Viral Dis Lab, Kunming, Yunnan, Peoples R China;[4]Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou, Peoples R China;[5]Natl Engn Res Ctr Freshwater Fisheries, Beijing Fisheries Res Inst, Beijing, Peoples R China;[6]Shandong First Med Univ & Shandong Acad Med Sci, Shandong Univ, Key Lab Etiol & Epidemiol Emerging Infect Dis, Jinan, Shandong, Peoples R China

第一机构：China CDC, Natl Inst Viral Dis Control & Prevent, 100 Ying Xin St, Beijing 100052, Peoples R China

通信机构：[1]corresponding author), China CDC, Natl Inst Viral Dis Control & Prevent, 100 Ying Xin St, Beijing 100052, Peoples R China.

年份：2021

卷号：10

期号：1

起止页码：1683

外文期刊名：EMERGING MICROBES & INFECTIONS

收录：;Scopus(收录号：2-s2.0-85113742899);WOS:【SCI-EXPANDED(收录号:WOS:000687850900001)】；

基金：This work was supported by National Science and Technology Major Project of China (2018ZX10305409-004-002), Basic Research Projects of Yunnan Province (2019FA015) and Yunnan Science and Technology Talents and Platform Plan (2018HB046).

语种：英文

外文关键词：SARS-CoV-2; bats; coronaviruses; recombination; evolution

摘要：At the end of 2019, A new type of beta-CoV, SARS-CoV-2 emerged and triggered the COVID-19 pandemic, which spread overwhelmingly around the world in less than a year. However, the origin and direct ancestral viruses of SARS-CoV-2 remain unknown. RaTG13, a novel coronavirus found in bats in China's Yunnan Province, is the closest relative virus of the SARS-CoV-2 identified so far. In this study, a new SARS-CoV-2 related virus, provisionally named PrC31, was discovered in Yunnan province by retrospectively analyse bat next generation sequencing (NGS) data of intestinal samples collected in 2018. PrC31 shared 90.7% and 92.0% nucleotide identities to the genomes of SARS-CoV-2 and the bat SARSr-CoV ZC45, respectively. Sequence alignment of PrC31 showed that several genomic regions, especially orf1a and orf8 had the highest homology with those corresponding genomic regions of SARS-CoV-2 than any other related viruses. Phylogenetic analysis indicated that PrC31 shared a common ancestor with SARS-CoV-2 in evolutionary history. The differences between the PrC31 and SARS-CoV-2 genomes were mainly manifested in the spike genes. The amino acid homology between the receptor binding domains of PrC31 and SARS-CoV-2 was only 64.2%. Importantly, recombination analysis revealed that PrC31 underwent multiple complex recombination events (including three recombination breakpoints) involving the SARS-CoV and SARS-CoV-2 sub-lineages, indicating that PrC31 evolved from yet-to-be-identified intermediate recombination strains. Combined with previous studies, it is revealed that the beta-CoVs may possess a more complex recombination mechanism than we thought.