文献类型：期刊文献

英文题名：Highly selective peptide-based fluorescent probe with aggregation induced emission (AIE) for detection of chondroitin sulfate and its application in living cells and zebrafish imaging

作者：Cao, Xinlin[1];Li, Shiyang[1];Pu, Chunmei[1];Deng, Weiliang[1];Wang, Peng[1,2];An, Yong[1,3]

第一作者：Cao, Xinlin

通信作者：Wang, P[1];An, Y[1]

机构：[1]China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Shida Rd 1, Nanchong 637009, Peoples R China;[2]Xihua Univ, Sichuan Engn Res Ctr Mol Targeted Diagnost & Thera, Dept Chem, Chengdu 610039, Peoples R China;[3]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730030, Gansu, Peoples R China

第一机构：China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Shida Rd 1, Nanchong 637009, Peoples R China

通信机构：[1]corresponding author), China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Shida Rd 1, Nanchong 637009, Peoples R China.

年份：2025

卷号：336

外文期刊名：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

收录：;EI(收录号：20251118052850);Scopus(收录号：2-s2.0-86000727164);WOS:【SCI-EXPANDED(收录号:WOS:001447169300001)】；

基金：This work was supported by the National Natural Science Foundation of China (NO. 22206154, 82303568), the project funds of Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province (No.CSPC202401), the project funds of Sichuan Engineering Research Center for Molecular Targeted Diagnostic & Therapeutic Drugs (XFZBXZLYW2024-005).

语种：英文

外文关键词：AIE characteristic; Tetrapeptide; Fluorescent probe; Chondroitin sulfate; Bioimaging

摘要：Chondroitin sulfate (CS), as a kind of acid mucosaccharide with natural activity, has shown various physiological activities in human body due to its advantages of high biocompatibility, good degradability and little side effects. Herein, a novel and simple peptide probe (TPE-ASRH) based on tetraphenylethene (TPE) and tetrapeptide (AlaSer-Arg-His-NH2) was designed and synthesized. TPE-ASRH displayed remarkable aggregation induced emission (AIE) characteristic in DMSO/water binary mixture. Based on electrostatic attraction, TPE-ASRH displayed highly selective and sensitive detection to chondroitin sulfate with large Stokes shift (156 nm), and the limit of detection (LOD) for chondroitin sulfate was calculated to be 0.11 nM based on 3 sigma/k. In addition, the colour change of TPE-ASRH was observed significantly from midnightblue to steelblue after adding chondroitin sulfate using naked eyes under 365 nm UV irradiation. Meanwhile, TPE-ASRH was able to achieve a rapid response to chondroitin sulfate (less than 30 s) with a pH response range of 3-12, which indicated that TPE-ASRH can detect chondroitin sulfate rapidly under physiological conditions. The response mechanism of TPE-ASRH to chondroitin sulfate was demonstrated using Zeta particle size and potential, UV-vis titration spectroscopy, FTIR