文献类型：期刊文献

中文题名：菖蒲郁金汤对多发性抽动症模型大鼠单胺类神经递质的影响

英文题名：Effect of Changpu Yujin Decoction on monoamine neurotransmitters in tourette syndrome model rats

作者：高汉媛[1];王维红[2];李小芹[1];杨新强[1];王继芳[1];尚菁[1];史正刚[1]

第一作者：高汉媛

机构：[1]甘肃中医药大学,兰州73000;[2]甘肃省妇幼保健院,兰州730050

第一机构：甘肃中医药大学

年份：2017

卷号：33

期号：3

起止页码：135

中文期刊名：中药药理与临床

外文期刊名：Pharmacology and Clinics of Chinese Materia Medica

收录：北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：甘肃省中药现代制药工程研究院资助项目;项目编号:No.YWW-2014005

语种：中文

中文关键词：菖蒲郁金汤;多发性抽动症;单胺类神经递质

外文关键词：Changpu Yujin Decoction（菖蒲郁金汤） ; Tourette Syndrome ;monoamine neurotransmitters

摘要：目的:观察菖蒲郁金汤对多发性抽动症(TS)模型大鼠单胺类神经递质的影响,探讨其治疗多发性抽动症的作用机理。方法:72只雄性SD大鼠随机分为空白组12只,造模组60只,造模组大鼠腹腔注射亚氨基二丙腈(IDPN)制备多发性抽动症动物模型,空白组大鼠腹腔注射等体积的生理盐水,每天1次,连续7天,造模完成后,造模组大鼠表现出不同程度的运动行为及刻板行为,评分均≥2分,表明造模成功。造模成功后,将造模组大鼠再次分为:模型组、泰必利组、菖蒲郁金汤77.28g/kg、38.64g/kg、19.32g/kg组,各12只。从造模完第2天开始给予灌胃,泰必利组给予泰必利(3.194mg/ml)混悬液,菖蒲郁金汤77.28g/kg、38.64g/kg、19.32g/kg组分别给予相应浓度的菖蒲郁金汤(5.152、2.576、1.288g生药/ml)药液,空白组与模型组给予等体积纯净水,每天1次,连续给药4周。在最后一次给药后24 h采集标本,用ELISA法检测大鼠脑组织及血浆多巴胺(DA)、去甲肾上腺素(NE)、5-羟色胺(5-HT)及高香草酸(HVA)水平。结果:(1)模型组大鼠纹状体DA含量明显下降,脑干5-HT含量明显升高;治疗后泰必利组大鼠纹状体DA含量明显升高,菖蒲郁金汤77.28g/kg组大鼠纹状体DA、HVA含量及脑干5-HT含量明显升高。(2)模型组大鼠血浆HVA含量明显降低;治疗后,泰必利组大鼠血浆HVA含量明显升高,菖蒲郁金汤77.28g/kg、38.64g/kg组大鼠血浆HVA含量明显升高,菖蒲郁金汤77.28g/kg、38.64g/kg、19.32g/kg组大鼠血浆5-HT含量明显升高。结论:单胺类神经递质间的平衡失调可能与多发性抽动症发病有关,而菖蒲郁金汤可能具有调节单胺类神经递质间平衡的作用。
Objective： Through observing the effects of Changpu Yujin （CY） decoction on the monoamine neurotransmitters＇content in brain tissue and plasma of Tourette Syndrome （TS） model rats, this paper discusses its regulation mechanism of CY decoction on the monoamine neurotransmitters of TS model rats. Method： 72 male SD rats were randomly divided into the blank group with 12 rats and the modeling group with 60 rats. The rats in the modeling group were intraperitoneally injected with IDPN, and the rats in the blank group were intraperitoneally injected with isovolumetric normal saline , once a day for 7 days. After modeling was complete, the rats in the modeling group appeared different levels of motor behavior and stereotyped behavior and the scores were ≥2 points, which indicated a successful modeling. Next, the rats in modeling group were randomly divided into model group, tiapride group and CY decoction （77.28g/kg, 38.64g/kg, 19.32g/kg） groups once again with 12 rats in each group respectively. After modeling success, the rats in the tiapride group were given tiapride suspension with 3. 194mg/m1, and the rats in the CY decoction （77.28g/kg, 38.64g/kg, 19.32g/kg） groups were given the CY decoction with corresponding concentration： 5. 152, 2. 576 and 1. 288g raw medicine/ml respectively. The rats in the blank group and model group were given isovolumetric pure water gavage, once a day for 4 weeks. After the intragastric administration for 28 days, the samples were collected 24 hours later. The ELISA method was used to detect the contents of DA, NE, 5-HT and HVA in brain tissue and plasma. Results ： （ 1 ） Compared with the blank group, the DA content in striatum of the rats in the model group had declined significantly （ P 〈 0.01 ）, and the 5-HT content in brain stem of the rats in the model group had increased obviously （P 〈 0.01 ）. Compared with the model group, the content of DA in striatum of the rats in the tiapride group had increased significantly （ P 〈 0.05） ; the content of DA and HVA in striatum and 5-HT in brain stem of the rats in the CY decoction （77.28g/kg） group had increased obviously （ P 〈 0.05, P 〈 0.05 and P 〈 0.01 respectively）. （2） Compared with the blank group, the HVA content in plasma of the rats in the model group had declined significantly （ P 〈 0.01 ）. Compared with the model group, the HVA content in plasma of the rats in the tiapride group had increased obviously （ P 〈 0.05 ） ; the HVA content in plasma of the rats in the CY decoction （77.28g/kg, 38.64g/kg） groups had increased obviously （ P 〈0.01 and P 〈0.05 respectively） ; the 5-HT content in plasma of the rats in the CY decoction （77.28g/kg, 38.64g/kg, 19.32g/kg） groups had increased obviously （ P 〈0.01, P 〈0.01 and P 〈 0.01 respectively）. Conclusions： The disequilibrium among monoamine neurotransmitters may be related to the pathogenesis of TS, and the CY decoction may have the effect of regulating the balance among monoamine neurotransmitters.