文献类型：期刊文献

中文题名：参苓白术散对脾虚湿困型溃疡性结肠炎大鼠结肠IL-13,IL-23及COX-2,CREB表达的影响

英文题名：Effect of Shenling Baizhu San on Expressions of IL-13,IL-23 and COX-2,CREB in Ulcerative Colitis Rats with Spleen Deficiency and Dampness

作者：丁凌辉[1];贾育新[1];成映霞[1];马文[2];段永强[1];明海霞[1];万生芳[1];程小丽[1]

第一作者：丁凌辉

机构：[1]甘肃中医药大学基础医学院,兰州730000;[2]甘肃省人民医院,兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2018

卷号：24

期号：11

起止页码：67

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金项目(81360518)

语种：中文

中文关键词：参苓白术散;溃疡性结肠炎;白细胞介素-13(IL-13);白细胞介素-23(IL-23);环氧合酶-2(COX-2);cAMP反应元件结合蛋白(CREB)

外文关键词：Shenling Baizhu San;ulcerative colitis;interleukin-13 （IL-13）;interteukin-23 （IL-23）;cyclooxygenase-2 （COX-2）;CAMP response element binding protein （CREB）

摘要：目的：观察参苓白术散对脾虚湿困型溃疡型结肠炎大鼠结肠组织白细胞介素（IL）-13,IL-23及环氧合酶-2（COX-2）,cAMP反应元件结合蛋白（CREB）表达的影响,探究其对溃疡性结肠炎作用机制。方法：将48只SPF级Wistar大鼠随机分为6组,分别是空白组、模型组、参苓白术散高、中、低剂量组和美沙拉秦组,每组8只,雌雄各半。除空白组,其余各组采用病证结合法复制脾虚湿困型溃疡性结肠炎大鼠模型,即饮食、环境干预等措施联合2,4-二硝基苯磺酸灌肠。造模成功后,参苓白术散高、中、低剂量组分别按照24,12,6 g·kg^-1·d^-1,美沙拉秦组0.2 g·kg^-1·d^-1进行灌胃。连续灌胃21 d后采用酶联免疫吸附法（ELISA）检测大鼠结肠组织IL-13,IL-23含量;实时荧光定量聚合酶链式反应法（Real-time PCR）检测结肠组织IL-13,IL-23,COX-2,CREB mRNA表达;蛋白免疫印迹法（Western blot）检测结肠组织COX-2,CREB蛋白表达量。结果：与空白组比较,模型组大鼠结肠IL-13含量和IL-13 mRNA表达水平明显降低（P〈0.05）,IL-23含量和IL-23 mRNA升高（P〈0.05）,COX-2,CREB mRNA及蛋白相对表达量升高（P〈0.05）。与模型组比较,参苓白术散（高、中、低剂量）组大鼠结肠IL-13含量和IL-13 mRNA表达均升高,尤以参苓白术散高剂量组最显著（P〈0.05）,IL-23含量和IL-23 mRNA降低（P〈0.05）,COX-2,CREB mRNA表达及蛋白相对表达量降低（P〈0.05）。结论：参苓白术散可能调节脾虚湿困型溃疡性结肠炎大鼠结肠IL-13,IL-23及COX-2,CREB的表达,减少结肠的炎症反应。
Objective： To observe the effect of Shenling Baizhu San on the expressions of interleukin(IL）-13,IL-23 and cyclooxygenase-2(COX-2）,CAMP response element binding protein(CREB） in ulcerative colitis rats with spleen deficiency and dampness,in order to explore its mechanism. Method： Totally 48 SPF Wistar rats were randomly divided into 6 groups： blank group,model group,high,medium and low-dose Shenling Baizhu San groups,and mesalazine group,with 8 rats in each group,half male and half female. Except for blank group,the rats in the other groups were included into the saphenous ulcerative colitis model by the combination of disease and syndrome,including diet,environmental intervention and 2,4-dinitrobenzene sulfonic acid enema.After successful modeling,Shenling Baizhu San group was respectively treated with the drug at high dose,middle dose and low dose(24,12,6 g·kg^-1·d^-1）,and mesalazine group was treated with the drug(0. 2 g·kg^-1·d^-1） for gavage. After 21 days of treatment,the levels of IL-13 and IL-23 in colonic tissues were detected by enzyme-linked immunosorbent assay(ELISA）; mRNA expressions of IL-13,IL-23,COX-2,CREB in colonic tissues were detected by Real-time PCR; the protein expressions of COX-2,CREB in colonic tissues were detected by Western blot. Result： Compared with the blank group,the model group rats＇ IL-13 content and IL-13 mRNA expression in colon were significantly decreased(P〈0. 05）,IL-23 content and IL-23 mRNA expression increased(P〈0. 05）,COX-2,CREB mRNA expression and relative protein expression increased(P〈0. 05）. Compared with the model group,IL-13 and IL-13 mRNA expressions in the colonic group were significantly higher than the rats treated with Shenling Baizhu San(high,medium and low doses）,especially in the high-dose group(P〈0. 05）,IL-23 content and IL-23 mRNA expression decreased(P〈0. 05）,and COX-2,CREB mRNA expressions and relative protein expression decreased(P〈0. 05）. Conclusion： Shenling Baizhu San can strengthen spleen Qi and excrete moisture,which may regulate the expressions of IL-13,IL-23 and COX-2,CREB in the colon of rats with spleen deficiency and dampness,so as to treat colonic ulcer.