文献类型：期刊文献

中文题名：植物生长调节剂壮根灵对党参药材中党参炔苷含量的影响

英文题名：Influence of plant growth regulator-Zhuanggenling on content of lobetyolin in crude Dangshen(Radix Codonopsis pilosulae)

作者：李成义[1];魏学明[1];李硕[1];李越峰[1];周海燕[2];王明伟[1]

第一作者：李成义

机构：[1]甘肃中医学院药学系;[2]中国药材公司

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2011

卷号：34

期号：11

起止页码：766

中文期刊名：北京中医药大学学报

外文期刊名：Journal of Beijing University of Traditional Chinese Medicine

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：甘肃省重大科技专项资助项目(No.1002FKDA048);国家公益性行业科研专项基金资助项目(No.200807020)

语种：中文

中文关键词：党参;壮根灵;党参炔苷;高效液相

外文关键词：Dangshen（Radix Codonopsis pilosulae）; Zhuanggenling; lobetyolin; HPLC

摘要：目的考察使用壮根灵对党参中党参炔苷含量影响,通过比较说明使用壮根灵对党参品质的影响。方法用高效液相色谱法,色谱柱:ZORBAX Edipse XDB-C18柱(250 mm×4.60 mm5,μm);流动相:乙腈-水溶液(28∶72)(V/V);流速:1.0 mL/min;进样量:10μL;柱温:30℃;检测波长:269 nm。结果 7个不同产地党参中使用壮根灵后党参中党参炔苷含量均明显下降。结论通过SPSS 16.0统计分析,壮根灵对药材中活性成分积累有显著性影响,在党参药材规范化种植过程中应慎重使用壮根灵。
Objective To investigate the influence of plant growth regulator-Zhuanggenling on the content of lobetyolin in crude Dangshen（Radix Codonopsis pilosulae）,and explain the effect of Zhuanggenling on the quality of Dangshen.Methods The HPLC procedure was performed on the chromatographic column of ZORBAX Edipse XDB-C18（250 mm×4.60 mm,5 μm）,and the mobile phase was acetonitrile and water solution（28：72,V/V）.The flow velocity was 1.0 mL/min,sample size,10 μL,temperature,30 ℃ and the detection wavelength,269 nm.Results The content of lobetyolin decreased significantly in Dangshen produced from 7 areas after using Zhuanggenling.Conclusion Zhuanggenling has significant influence on the accumulation of active constituents in Dangshen after SPSS 16.0 statistical analysis,so which should be used carefully in the standardized plantation of Dangshen.