文献类型：期刊文献

英文题名：Butylphthalide ameliorates airway inflammation and mucus hypersecretion via NF-kappa B in a murine asthma model

作者：Wang, Zhiwang[1,2];Yao, Nan[2];Fu, Xiaoyan[2];Wei, Lingxia[2];Ding, Maopeng[2];Pang, Yarong[2];Liu, Dongling[2];Ren, Yuan[1,2];Guo, Mei[2]

第一作者：王志旺

通信作者：Wang, ZW[1];Guo, M[1]

机构：[1]Gansu Univ Chinese Med, Key Lab Pharmacol & Toxicol Tradit Chinese Med Ga, Lanzhou, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Sch Pharm, Lanzhou, Gansu, Peoples R China

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Pharm Lanzhou, Lanzhou Campus,35 Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2019

卷号：76

外文期刊名：INTERNATIONAL IMMUNOPHARMACOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000495488900020)】；

基金：We thank the Animal Experiment Centre of Gansu University of Chinese Medicine for the experimentation on animals. We gratefully acknowledge financial support from the National Natural Science Foundation of China (81460668), the Natural Science Foundation of Gansu Province (1606RJZA011, 1310RJZA086) and the foundation of the Key Laboratory of Pharmacology and Toxicology for Traditional Chinese Medicines of Gansu Province, Gansu College of Chinese Medicine (ZDSYS-KJ-2018-001).

语种：英文

外文关键词：Butylphthalide; Asthma; Airway inflammation; Mucus hypersecretion; Nuclear transcription factor-kappa B

摘要：Butylphthalide (NBP) is a phthalide compound contained in Angelicae Sinensis Radix which is one of the most widely used traditional Chinese medicines. This study aims to explore the therapeutic effect of NBP on airway inflammation, mucus hypersecretion and their possible mechanism in asthma mice. BALB/c mice were sensitized and challenged with ovalbumin (OVA) for establishment of asthma model and then treated with NBP during day 22-77. The pulmonary function of the mice was determined, and the pathology of lung tissue and goblet cell hyperplasia were observed through analyzing inflammation scores and goblet cell percentage, respectively. Cytokine IL-4, IL-8, IL-13 and tumor necrosis factor-alpha (TNF-alpha) in bronchoalveolar lavage fluid (BALF) and total immunogloblin E (T-IgE) and OVA-specific IgE in serum were examined by enzyme-linked immunosorbent assay (ELISA). The expressions of Mucin 5AC (Muc5ac) and nuclear transcription factor-kappa B (NF-kappa B) in lung tissues were evaluated by immunohistochemistry, western blot and real-time polymerase chain reaction (RT-PCR). The results show that 50 mg/kg NBP significantly reduced OVA-induced increase in inflammation scoring, goblet cell percentage and mucus secretion of airway tissue, and improved the pulmonary function. NBP could also decrease IL-4, IL-8 IL-13, and TNF-alpha in BALF and T-IgE and OVA-specific IgE in serum. The expression of Muc5ac and NF-kappa B in lung tissue was significantly down-regulated after NBP treatment. This study suggested that NBP may effectively inhibit airway inflammation and mucus hypersecretion in asthma by modulating NF-kappa B activation.