文献类型：期刊文献

中文题名：香砂六君子对脾胃虚弱型功能性消化不良大鼠十二指肠低度炎症的作用研究

英文题名：Study of Xiangsha Liujunzi on low-grade duodenal inflammation in rats with functional dyspepsia of spleen stomach weakness

作者：赵琳娜[1,2];白敏[1,2];李润法[1,2];刘梦雅[1,2];段永强[3,4,5];高晗[6];安耀荣[1];成映霞[3,4,5]

第一作者：赵琳娜

机构：[1]甘肃中医药大学基础医学院,甘肃兰州730000;[2]甘肃省实验动物行业技术中心,甘肃兰州730000;[3]宁夏医科大学中医学院,宁夏回族自治区银川750004;[4]宁夏区域高发病中医药防治教育部重点实验室,宁夏回族自治区银川750004;[5]宁夏区域高发病中西医结合研究重点实验室,宁夏回族自治区银川750004;[6]石家庄傅山肿瘤医院中医内科,河北石家庄050000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2023

卷号：39

期号：15

起止页码：2198

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：宁夏回族自治区科技厅重点研发引才专项基金资助项目(2022BSB03080);甘肃中医药大学研究生创新创业基金资助项目(2022CX06)。

语种：中文

中文关键词：香砂六君子汤;功能性消化不良;低度炎症;Nod结构域样受体3

外文关键词：Xiangsha Liujunzi decoction;functional dyspepsia;low-grade inflammation;Nod domain-like receptor 3

摘要：目的基于Nod结构域样受体3(NLRP3)活化探讨香砂六君子(XSLJZ)对脾胃虚弱型功能性消化不良(FD)大鼠十二指肠低度炎症的干预效应及机制。方法将SD大鼠随机分为空白组与造模组,通过复合造模法(碘乙酰胺灌胃+游泳力竭+饥饱失常)建立脾胃虚弱型FD大鼠,并进行模型验证。将成功建模的脾胃虚弱型FD大鼠随机分为模型组和低(6 g·kg^(-1))、高(12 g·kg^(-1))剂量实验组。给药2周后,检测各组大鼠体质量及进食量并测算其胃排空率及肠推进率,以酶联免疫吸附试验法测定各组大鼠血清中白细胞介素(IL)-1β、IL-18的含量,以蛋白质印迹法及免疫荧光染色(IF)法检测NLRP3、胱天蛋白酶-1(caspase-1)、孔蛋白Gasdermoh-D(GSDMD)蛋白的表达水平。结果给药后,空白组、模型组和低、高剂量实验组的体质量分别为(390.50±34.30)、(305.20±33.10)、(338.30±34.84)和(352.40±33.40)g,3 h进食量分别为(14.51±1.36)、(10.31±1.37)、(12.99±1.34)和(13.77±1.55)g,胃排空率分别为(56.75±5.05)%、(29.50±5.02)%、(46.40±6.83)%和(47.30±9.00)%,肠推进率分别为(86.66±12.26)%、(35.38±11.53)%、(60.29±13.26)%和(75.09±10.10)%,IL-1β含量分别为(29.27±4.79)、(47.18±8.98)、(38.04±2.92)和(31.22±4.97)pg·mL^(-1),IL-18含量分别为(23.12±5.47)、(110.62±18.33)、(72.99±8.40)和(40.36±6.83)ng·mL^(-1);模型组的上述指标与空白组和低、高剂量实验组比较,差异均有统计学意义(P<0.05,P<0.01)。结论XSLJZ可以有效抑制十二指肠低度炎症,其机制可能与NLRP3活化有关。
Objective To investigate the effect and molecular mechanism of Xiangsha Liujunzi(XSLJZ)on low-grade duodenal inflammation in rats with functional dyspepsia(FD)of spleen-stomach weakness based on Nod domain-like receptor 3(NLRP3)activation.Methods SD rats were randomly divided into blank group and model group.FD rats with weakness of spleen and stomach were reconstructed by compound modeling method(iodoacetamide gavage+swimming exhaustion+hunger-satiety disorder),and the model was verified.FD rats with spleen and stomach weakness were randomly divided into model group and low(6 g·kg^(-1)),high(12 g·kg^(-1))experimental dose groups.After 2 weeks of administration,the body weight and food intake of rats in each group were measured and their gastric emptying rate and intestinal propulsion rate were measured.The contents of IL-1βand IL-18 in the serum of rats in each group were determined by enzyme-linked immunosorbent assay(ELISA).The expression levels of NLRP3,caspase-1 and GSDMD protein were detected by Western blot(WB)and immunofluorescence staining(IF).Results The body weight of blank group,model group and low,high dose experimental groups were(390.50±34.30),(305.20±33.10)(338.30±34.84)and(352.40±33.40)g;the 3-hour food intake were(14.51±1.36),(10.31±1.37),(12.99±1.34)and(13.77±1.55)g;the gastric emptying rate were(56.75±5.05)%,(29.50±5.02)%,(46.40±6.83)%and(47.30±9.00)%;the intestinal propulsion rate were(86.66±12.26)%,(35.38±11.53)%,(60.29±13.26)%and(75.09±10.10)%;IL-1βcontents were(29.27±4.79),(47.18±8.98),(38.04±2.92)and(31.22±4.97)pg·mL^(-1);IL-18 contents were(23.12±5.47),(110.62±18.33),(72.99±8.40)and(40.36±6.83)pg·mL^(-1).The above indexes of model group were statistically significant compared with blank group and low,high-dose experimental groups(P<0.05,P<0.01).Conclusion XSLJZ can effectively inhibit low-grade duodenal inflammation,and the mechanism may be related to NLRP3 activation.