文献类型：期刊文献

中文题名：早期抽薹对当归根际土壤微环境的影响

英文题名：Effect of Premature Bolting on the Rhizosphere Soil Microenvironment of Angelica sinensis

作者：谢田朋[1];张佳宁[1];董永骏[2];张建[1];景明[1]

第一作者：谢田朋

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃祁连山国家级自然保护区管护中心,张掖734000

第一机构：甘肃中医药大学

年份：2023

卷号：39

期号：7

起止页码：206

中文期刊名：生物技术通报

外文期刊名：Biotechnology Bulletin

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金项目(82160714);甘肃省“三区”人才计划项目(219);甘肃省青年博士基金项目(2021QB-075);甘肃省产业支撑计划项目(2021CYZC-13);兰州市指导性计划项目(2022-5-167);甘肃中医药大学科创基金重点项目(2019KCZD-3,2020KCZD-3)。

语种：中文

中文关键词：当归抽薹;根际土壤;土壤性质;细菌属;代谢物

外文关键词：Angelica sinensis bolting;rhizosphere soil;soil properties;bacterial community;metabolite

摘要：通过探讨根际土壤微环境在当归抽薹时的变化,尝试为解决当归早期抽薹问题提供新的思路。以当归为研究对象,测定抽薹与未抽薹当归的根际土壤理化性质,利用16S rDNA和GC-MC技术测定根际土壤细菌群落及代谢组变化,并研究根际土壤性质、细菌群落及代谢物之间在当归抽薹现象中的关联性。结果显示,抽薹当归根际土壤中硝态氮含量较未抽薹当归明显升高。根际土壤细菌群落多样性及优势菌群在抽薹和未抽薹当归间并无差异,细菌群落较为相似,但10个非优势菌属在组间存在显著差异。抽薹当归根际土壤中有66种代谢物与未抽薹当归存在显著差异,其中52种明显上调,14种明显下调。差异代谢物主要富集在氨基酸代谢、异种生物降解和代谢、脂质代谢等7条相关通路上。硝态氮含量、Phaselicystis属和Rubellimicrobium属与抽薹当归差异代谢物显著负相关(P<0.05),硝态氮主要与不饱和脂肪酸的生物合成显著负相关,Phaselicystis属和Rubellimicrobium属主要与氨基苯甲酸酯降解、精氨酸和脯氨酸代谢显著负相关。当归早期抽薹与根际土壤硝态氮含量上升存在关联,同时当归早期抽薹会引起根际土壤代谢物和非优势菌群的相对丰度显著变化。根际土壤中硝态氮含量上升与Nitrosomonas属的相对丰度变化可能存在联系,但仍需进一步研究论证。
The changes of rhizosphere soil microenvironment during Angelica sinensis bolting were explored to provide new ideas for solving the problem of premature bolting.A.sinensis was taken as the research object,the soil properties,bacterial community and metabolite in the rhizosphere soil were determined by 16S rDNA and GC-MC techniques in bolting and unbolting A.sinensis,and the correlation among them was studied.The results show that nitrate nitrogen content in the rhizosphere soil of bolting groups were significantly higher than those of unbolting ones.There was no difference between bolting and unbolting groups in the diversity of rhizosphere soil bacterial community and the structure of dominant bacteria,thus the bacterial community was alike.However,there were significantly differences among 10 non-dominant genera.There were 66 metabolites in the rhizosphere soil of bolting groups,which were different from the unbolting ones,52 of which were significantly up and 14 significantly down.The differential metabolites were mainly concentrated in 7 related pathways of amino acid metabolism,heterogeneous biodegradation and metabolism,and lipid metabolism.The nitrate nitrogen content,Phaselicystis,Rubellimicrobium were significantly negatively correlated to the differential metabolites of bolting groups(P<0.05).The nitrate nitrogen was mainly negatively related to the biosynthesis of unsaturated fatty acids,while Phaselicystis and Rubellimicrobium were mainly negatively related to the degradation of aminobenzoate,arginine and proline metabolism.The premature bolting of A.sinensis was related to the increase of nitrate nitrogen content in the rhizosphere soil,and the premature bolting will cause significant changes in the relative abundance of non-dominant bacteria and metabolites in the rhizosphere soil.The increase of nitrate nitrogen content may be related to the change of the relative abundance of Nitrosomonas,but further research is still needed.