详细信息
Effect of Two Kinds of Compound Rhizosphere Growth-promoting Bacteria on Growth, Physiological Characteristics and Pharmacodynamic Components of Angelicae Sinensis Radix
文献类型:期刊文献
英文题名:Effect of Two Kinds of Compound Rhizosphere Growth-promoting Bacteria on Growth, Physiological Characteristics and Pharmacodynamic Components of Angelicae Sinensis Radix
机构:[1]College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, 730000, China;[2]Gansu Key Laboratory of Microbial Resources Development and Utilization, Institute of Biology, Gansu Academy of Sciences, Lanzhou, 730000, China;[3]Northwest Collaborative Innovation Center of Traditional Chinese Medicine (TCM) and Tibetan Medicine, Gansu Key Laboratory Breeding Base for TCM Quality and Standard Research, Lanzhou, 730000, China
第一机构:甘肃中医药大学药学院(西北中藏药协同创新中心办公室)
年份:2022
卷号:28
期号:8
起止页码:131
外文期刊名:Chinese Journal of Experimental Traditional Medical Formulae
收录:Scopus(收录号:2-s2.0-85129724557)
语种:英文
外文关键词:Angelicae Sinensis Radix; Bacillus; endogenous hormones; pharmacodynamic composition; photosynthesis; plant growth-promoting rhizobacteria; Pseudomonas
摘要:Objective: To study the effects of foliar spraying of two kinds of compound rhizosphere growth-promoting agents on the growth and physiological characteristics of Angelicae Sinensis Radix(ASR),as well as the pharmacodynamic components,in order to lay a foundation for providing functional microbial agents for ecological cultivation of ASR. Method:The compound growth-promoting agents T1(Pseudomonas CBS5, CBS7 and CBSB)and T2(Bacillus 5C1,5C5 and 5C7)with the concentration of 1×108 CFU·mL-1 were sprayed on the leaf surface of the field,and the sterile potato glucose broth medium was used as the control (CK). The plant growth indexes of ASR were measured by conventional methods, the photosynthetic physiological indexes of ASR were measured by portable photosynthetic measurement system,the enzyme activities of plants and microorganisms were measured by kit method,and the endogenous hormone levels were analyzed by ultra-performance liquid chromatography tandem mass spectrometry. The contents of ferulic acid,senkyunolide I,coniferyl ferulate,senkyunolide A and Z-ligustilide were determined by high performance liquid chromatography. Result: Compared with CK,the two compound inoculants could promote the growth of ASR and increase the biomass,increase the leaf net photosynthetic rate,stomatal conductance,intercellular CO2 concentration,transpiration rate,increase catalase,peroxidase,superoxide dismutase,polyamine oxidase, diamine oxidase and polyphenol oxidase enzyme activities,increase endogenous jasmonic acid,cytokinin and gibberellin levels in plants,increase the contents of ferulic acid,senkyunolide A and Z-ligustilide,reduce the contents of malondialdehyde and abscisic acid,and reduce the incidence of root rot. Conclusion: Foliar spraying of two kinds of rhizosphere compound growth-promoting agents can promote the growth,photosynthesis and stress resistance of ASR, and can improve the quality of ASR in different degrees. Comprehensive analysis shows that T1 treatment is better than T2 treatment in the growth-promoting and quality-enhancing of ASR. ? 2022, China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica. All rights reserved.
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