文献类型：期刊文献

英文题名：Mechanism of Xiaoqinglong Decoction in treatment of chronic obstructive pulmonary disease based on transcriptomics; [基于转录组学探讨小青龙汤治疗慢性阻塞性肺疾病的作用机制]

作者：Fu Z.; Wang Y.; Hei X.; Fu X.; Yang Z.; Wang X.

第一作者：Fu Z.

机构：[1]School of Public Health, Gansu University of Chinese Medicine, Lanzhou, 730000, China;[2]School of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou, 730000, China

第一机构：甘肃中医药大学公共卫生学院

通信机构：[1]School of Public Health, Gansu University of Chinese Medicine, Lanzhou, 730000, China;[1]School of Public Health, Gansu University of Chinese Medicine, Lanzhou, 730000, China|[10735e9d5e7087247e71b]甘肃中医药大学公共卫生学院;[10735]甘肃中医药大学;

年份：2025

卷号：56

期号：8

起止页码：2849

外文期刊名：Chinese Traditional and Herbal Drugs

收录：Scopus(收录号：2-s2.0-105003285157)

语种：英文

外文关键词：chronic obstructive pulmonary disease; cinnamaldehyde; cyclic adenosine monophosphate signaling pathway; ephedrine; inflammatory factors; transcriptomics; vascular endothelial growth factor; Xiaoqinglong Decoction

摘要：Objective To investigate the mechanism of Xiaoqinglong Decoction (小青龙汤) in treating chronic obstructive pulmonary disease (COPD) based on transcriptomics analysis. Methods Male C57BL/6 mice were randomly divided into control group, model group, dexamethasone (1 mg/kg) group, and Xiaoqinglong Decoction low-, medium-, high-dose (1.46, 0.73, 0.36 g/kg) groups, with eight mice in each group. Except for the control group, all other groups of mice were used to construct COPD models by intranasal infusion of tobacco extract combined with lipopolysaccharide. After continuous administration for four weeks, the general status of mice in each group were observed, hematoxylin-eosin (HE) staining was used to observe the pathological changes in lung tissue of mice, ELISA was used to measure the levels of inflammatory factors in serum; Transcriptome sequencing technology was used to analyze the gene expression profiles of lung tissues in control group, model group and Xiaoqinglong Decoction high-dose group, gene ontology (GO) function and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were performed on significantly differentially expressed genes; qRT-PCR and Western blotting were used to detect the expressions of genes and proteins related to cyclic adenosine monophosphate (cAMP) signaling pathway in lung tissue. Results Compared with control group, mice in model group showed poor general condition, significant pathological damage in lung tissue, and significantly increased levels of inflammatory factors in serum (P < 0.01); Compared with model group, the general condition and lung tissue damage of mice in each treatment group were improved to varying degrees, and the levels of inflammatory factors in serum were significantly reduced (P < 0.05, 0.01). Among them, Xiaoqinglong Decoction high-dose group and dexamethasone group had the most significant improvement effect. The transcriptome analysis results showed that the differentially expressed genes between control group and model group, as well as Xiaoqinglong Decoction high-dose group were widely involved in cAMP signaling pathways, neuroactive ligand-receptor interactions, circadian rhythms and other related signaling pathways. qRT-PCR and Western blotting results showed that compared with control group, the expressions of cAMP, PKA and CREB in lung tissue of mice in model group mice were significantly reduced (P < 0.01), while the expression of VEGF was significantly increased (P < 0.01); Compared with model group, Xiaoqinglong Decoction high-dose group significantly increased the expressions of cAMP, PKA and CREB in lung tissue of mice (P < 0.05, 0.01), and significantly decreased the expression of VEGF (P < 0.01). Conclusion Xiaoqinglong Decoction could significantly reduce inflammatory cell infiltration in the airways of COPD mice, alleviate lung airway remodeling, and treat COPD by regulating cAMP signaling pathway. ? 2025 Editorial Office of Chinese Traditional and Herbal Drugs. All rights reserved.