文献类型：期刊文献

英文题名：LncRNA FER1L4 suppressed cancer cell growth and invasion in esophageal squamous cell carcinoma

作者：Ma, W.[1];Zhang, C-Q.[2];Li, H-L.[3];Gu, J.[4];Miao, G-Y.[1];Cai, H-Y.[1];Wang, J-K.[1];Zhang, L-J.[1];Song, Y-M[3];Tian, Y-H.[3];Song, Y-H.[3]

第一作者：Ma, W.

通信作者：Zhang, CQ[1]

机构：[1]Gansu Prov Hosp, Dept Radiotherapy, Lanzhou, Gansu, Peoples R China;[2]Gansu Prov Hosp, Dept Radiol, Lanzhou, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Sch Clin Med, Basic Teaching & Res Off Clin Examinat, Lanzhou, Gansu, Peoples R China;[4]Gansu Univ Chinese Med, Sch Clin Med, Dept Physiol, Lanzhou, Gansu, Peoples R China

第一机构：Gansu Prov Hosp, Dept Radiotherapy, Lanzhou, Gansu, Peoples R China

通信机构：[1]corresponding author), Gansu Prov Hosp, Dept Radiol, Lanzhou, Gansu, Peoples R China.

年份：2018

卷号：22

期号：9

起止页码：2638

外文期刊名：EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES

收录：;Scopus(收录号：2-s2.0-85046953005);WOS:【SCI-EXPANDED(收录号:WOS:000432376300017)】；

基金：This study was supported by the Foundation of the Fundamental Scientific Research Funds for Colleges and Universities of Gansu Province (No. [2014]63-15); NSFC (Natural Science Foundation of China) Regional Science Funds Project (No. 81560093) and Gansu Provincial Science and Technology Department (No.17JR5RA037).

语种：英文

外文关键词：IncRNA FER1L4; Esophageal squamous cell carcinoma; Proliferation; Invasion

摘要：OBJECTIVE: To investigate the regulatory effect of long non-coding ribonucleic acid (IncRNA) FER1L4 on biological behaviors of esophageal squamous cell carcinoma (ESCC) cells, such as proliferation and invasion. PATIENTS AND METHODS: The expressions of FER1L4 were detected in 42 pairs of ESCC tissues and corresponding para-carcinoma tissues and 5 kinds of ESCC cell lines via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Polyethyleneimine (PEI) and liposomes were used for FER1L4 expression or interference elimination assays, respectively. The proliferation and invasion of ESCC cells were detected via MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), apoptosis assay, cell cycle assay, and transwell chamber. RESULTS: Results of qRT-PCR showed that, compared with that in normal tissues, FER1L4 was lowly expressed in ESCC tissues. Overexpression of FER1L4 could inhibit cell proliferation and invasion, promote apoptosis and increase the cell cycle distribution in G0/G1 phase. Knockout of FER1L4 could promote the proliferation and invasion of ESCC cells, inhibit apoptosis and decrease the cell cycle distribution in G0/G1 phase. CONCLUSIONS: FER1L4 is involved in the occurrence and development of ESCC and plays a key role as a tumor suppressor gene in ESCC.