文献类型：期刊文献

英文题名：Rapid screening and identification of anticoagulation component from carthami flos by two-dimensional thrombin affinity chromatography combined with HPLC-MS/MS

作者：Qiao, Yanru[1,2];Shi, Yingdi[1,2];Wu, Chen[3];Hou, Xiaofang[1,2];Pan, Xiaoyan[1];Deng, Zijie[1,2];Wang, Sicen[1,2]

第一作者：Qiao, Yanru

通信作者：Hou, XF[1];Wang, SC[2]

机构：[1]Xi An Jiao Tong Univ, Sch Pharm, Hlth Sci Ctr, 76 Yanta West Rd, Xian 710061, Shaanxi, Peoples R China;[2]Shaanxi Engn Res Ctr Cardiovasc Drugs Screening &, Xian, Peoples R China;[3]Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China

第一机构：Xi An Jiao Tong Univ, Sch Pharm, Hlth Sci Ctr, 76 Yanta West Rd, Xian 710061, Shaanxi, Peoples R China

通信机构：[1]corresponding author), Xi An Jiao Tong Univ, Sch Pharm, Hlth Sci Ctr, 76 Yanta West Rd, Xian 710061, Shaanxi, Peoples R China;[2]corresponding author), Xi An Jiao Tong Univ, Sch Pharm, 76 Yanta Wt Rd, Xian 710061, Shaanxi, Peoples R China.

年份：2021

卷号：44

期号：16

起止页码：3061

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20212610576110);Scopus(收录号：2-s2.0-85108818127);WOS:【SCI-EXPANDED(收录号:WOS:000667792400001)】；

基金：Financial support from the China Postdoctoral Science Foundation (2018M643681), the National Natural Science Foundation of Shaanxi Province (2017JQ8024, 2019SF-153), and Shaanxi Province Innovative Talent Promotion Plan (2019TD-017) are gratefully acknowledged.

语种：英文

外文关键词：anticoagulant component; carthami flos; high performance liquid chromatography; hydroxysafflor yellow A; thrombin affinity chromatography

摘要：Carthami flos, commonly known as Honghua in China, is the dried floret of safflower and widely acknowledged as a blood stasis promoting herb. The study aimed at investigating the relationship between thrombin and carthami flos through a high-performance thrombin affinity chromatography combined with a high-performance liquid chromatography-tandem mass spectrometry system. First, thrombin was immobilized on the glutaraldehyde-modified amino silica gel to prepare the thrombin affinity stationary phase, which was packed into a small column (1.0 x 2.0 mm, id) for recognizing the anticoagulant active components of carthami flos. The target component was enriched and analyzed by the high-performance liquid chromatography-tandem mass spectrometry system. Finally, hydroxysafflor yellow A was screened out and identified as the active component. The anticoagulant effects of hydroxysafflor yellow A were analyzed by anticoagulant experiments in vitro, and the interaction of hydroxysafflor yellow A with thrombin was investigated by the molecular docking method. The results proved that hydroxysafflor yellow A (30 mu g/mL, 0.05 mM) and carthami flos extract (30 mu g/mL) could prolong activated partial thrombin time and thrombin time by 50 and 11%, respectively. Moreover, hydroxysafflor yellow A exhibits a good hydrogen bond field and stereo field matching with thrombin. Overall, it was concluded that hydroxysafflor yellow A might exert an anticoagulation effect by interacting with thrombin and thus could be potential anticoagulant drugs for the prevention and treatment of venous thrombosis.