文献类型：期刊文献

中文题名：当归多糖及小分子提取物对TGF-β1诱导的HELF表达α-SMA、CTGF的影响

英文题名：Effects of Angelica polysaccharides and Angelica small molecules on expression of α-SMA,CTGF in human embryonic lung fibroblast cells induced by TGF-β1

作者：骆亚莉[1,2];安方玉[2];李能莲[1];刘永琦[2];李程豪[2];张艳辉[2];陈佳[3]

第一作者：骆亚莉

机构：[1]甘肃中医药大学基础医学院病理教研室,兰州730000;[2]甘肃中医药大学重大疾病分子医学与中医药防治研究重点实验室,兰州730000;[3]甘肃中医药大学公共卫生学院,兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2017

卷号：33

期号：3

起止页码：73

中文期刊名：中药药理与临床

外文期刊名：Pharmacology and Clinics of Chinese Materia Medica

收录：北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：甘肃省自然科学基金项目(1212RJZA077)

语种：中文

中文关键词：当归多糖;当归小分子提取物;转化生长因子-β1;人胚肺成纤维细胞;α-平滑肌肌动蛋白;结缔组织生长因子

外文关键词：Angelica polysaccharides（当归多糖） ; Angelica small molecules（当归小分子提取物） ; transforming growth factor-β1; hu-man embryonic lung fibroblast cells; α-SMA; CTGF

摘要：目的:观察当归多糖及小分子提取物对TGF-β1诱导的人胚肺成纤维细胞HELF的增殖及α-SMA、CTGF影响的作用。方法:建立肺纤维化细胞模型,分为空白组、模型组、12.5μg/ml当归多糖组、25μg/ml当归多糖组、50μg/ml当归多糖组、25μg/ml当归小分子提取物组、50μg/ml当归小分子提取物组、100μg/ml当归小分子提取物组。结合形态观察,检测细胞增殖、羟脯氨酸、MMP-9、TIMP-1含量及α-SMA、CTGF蛋白表达的变化。结果:与空白组比较,各组细胞吸光度值、羟脯氨酸、MMP-9、TIMP-1含量、CTGF、α-SMA蛋白表达均明显增高。与模型组比较,25μg/ml当归多糖组、50μg/ml当归小分子提取物组细胞吸光度值、羟脯氨酸含量、CTGF、α-SMA蛋白表达均降低,50μg/ml当归小分子提取物组MMP-9含量下降,25μg/ml当归多糖组、50μg/ml当归小分子提取物组TIMP-1含量均增高。形态学观察,模型组细胞数量增多,体积增大,生长旺盛。25μg/ml当归多糖组、50μg/ml当归小分子提取物组细胞数量较少,体积较小。结论:当归多糖及小分子提取物能抑制TGF-β1诱导的HELF增殖,减少羟脯氨酸的合成,其作用可能与下调α-SMA、CTGF表达,调节维持MMP-9/TIMP-1之间的平衡有关。
Objective： To investigate the effect of Angelica polysaccharide （AP） and small molecules on the expression ofα-SMA, CTGF induced by transforming growth factor-β1 （TGF-β1） in human embryonic lung fibroblast cells （HELF）. Methods： HELF cells were cultured in vitro and divided into the control group, the model group, 12.5 μg/ml Angelica Polysaccharide group, 25 μg/ml Angelica Polysaccharide group , 50μg/ml Angelica Polysaccharide group, 25 μg,/ml Angelica small molecules groups, 50 μml Angelica small molecules groups and 100μg/ml Angelica small molecules groups. Except for HELF morphology observation, we observed cell proliferation changes. Also contents of hydroxyproline, MMP-9 and TIMP-1 were detected by colorimetry and ELISA method respectively. The proteins expressions of α-SMA and CTGF were determined by Western-blot. Results： Compared with the control group, the cell absorbancy value （A）, contents of hydroxyproline, MMP-9 and TIMP-1, protein expressions of α-SMA, CTGF in other groups were all increased （ P 〈0. 05 or P 〈0. 01 ）. Compared with model group, A value, contents of hydroxyproline and protein expressions of α-SMA, CTGF were all lowered in 25 μg/ml Angelica Sinensis Polysaccharide and 50 μg/ml Angelica small molecular （ P 〈 0.05 or P 〈 0.01 ） groups. Contents of MMP-9 in 50 μg/ml small molecules group also decreased （ P 〈 0.05）, however contents of TIMP-1 in 25 μg/ml Angelica sinensis polysaccharide and 50μg/ml small molecules group both increased, compared with the model group （ P 〈 0.05 or P 〈 0.01 ）. Morphological changes showed that cells were much more and much bigger in the model group than in the control group, so cells growth were vigorous. Compared with the model group, the number of cells was less and volumes were smaller in 25 μg/ml Angelica Sinensis Polysaccharide and 50 μg/ml small molecular group. Conclusion： Angelica Polysaccharides, small molecules can inhibit the proliferation of embryonic lung fibroblasts induced by TGF-beta 1, reduce the synthesis of hydroxyproline. Their anti-fibrosis mechanisms may be related to lowering expression of α-SMA, CTGF and regulatinge balance between MMP-9/TIMP-1.