文献类型：期刊文献

英文题名：Huangqi Baihe Granules alleviate chemotherapy-induced intestinal mucositis in Drosophila and mice

作者：Kang, Qian[1];Xiu, Minghui[2];Wang, Xiaoqian[1];Wu, Jinhan[1];Wang, Yixuan[2];Liu, Yongqi[1,2];Cao, Wangjie[1];Gong, Hongxia[1];Li, Congyi[1];Shi, Jinghong[4];He, Jianzheng[1,2,3];Su, Yun[1,2]

第一作者：Kang, Qian

通信作者：He, JZ[1];Su, Y[1];He, JZ[2];Su, Y[2];He, JZ[3]

机构：[1]Gansu Univ Chinese Med, Treatment Tradit Chinese Med Res Gansu Coll & Univ, Prov Level Key Lab Mol Med Major Dis & Prevent, Lanzhou 730000, Peoples R China;[2]Minist Educ, Key Lab Dunhuang Med, Lanzhou 730000, Peoples R China;[3]Gansu Univ Chinese Med, Sci Res & Expt Ctr, Lanzhou 730000, Peoples R China;[4]Shaanxi Univ Chinese Med, Xianyang 712000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Treatment Tradit Chinese Med Res Gansu Coll & Univ, Prov Level Key Lab Mol Med Major Dis & Prevent, Lanzhou 730000, Peoples R China;[2]corresponding author), Minist Educ, Key Lab Dunhuang Med, Lanzhou 730000, Peoples R China;[3]corresponding author), Gansu Univ Chinese Med, Sci Res & Expt Ctr, Lanzhou 730000, Peoples R China.|[107359c91a78c5fd2e803]甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）;[10735]甘肃中医药大学;

年份：2026

卷号：16

期号：1

外文期刊名：AMB EXPRESS

收录：;Scopus(收录号：2-s2.0-105034933934);WOS:【SCI-EXPANDED(收录号:WOS:001722452000001)】；

基金：This work was supported by the Gansu Provincial Center for Traditional Chinese Medicine Research (No. Zyzx-2023-04), Gansu Natural Science Foundation (Nos. 23JRRA1202, 23JRRA1714 and 25JRRA1177).

语种：英文

外文关键词：Chemotherapy-induced intestinal mucositis (CIM); Huangqi Baihe Granules (HQBHG); NF-kappa B pathway; Nrf2/Keap1 pathway; Amino acid metabolism

摘要：Chemotherapy-induced intestinal mucositis (CIM) is a significant dose-limiting adverse effect of cancer treatment. Huangqi Baihe Granules (HQBHG) derived from Dunhuang's ancient medical texts could alleviate radiation brain injury and hypobaric hypoxia-induced acute lung injury. Here, the protective effect and mechanism of HQBHG against irinotecan (CPT-11)-induced intestinal mucositis were detected by using Drosophila melanogaster and mouse models. Oral administration of HQBHG could significantly ameliorate body injury caused by CPT-11, including increased survival rate, rescued locomotion, altered metabolic capacity, restoration of ovarian morphology in flies, and also alleviated body weight loss and diarrhea in mice. Meanwhile, HQBHG supplementation resumed intestinal length and gastrointestinal acid-based homeostasis, reduced epithelial cell death in CPT-11 treated flies. In CPT-11 treated mice, HQBHG increased the gut length, recovered the architecture of the mucosa, and increased the expressions of tight junction proteins (ZO-1 and occludin ). Mechanism study showed that HQBHG remarkably down-regulated the expression levels of NF-kappa B signaling, the levels of cytokines IL-1 beta and TNF-alpha, and intestinal ROS accumulation; whereas it significantly up-regulated the levels of IL-10, and the expression of the Keap1/Nrf2 signaling in the guts. In addition, integrated analysis of 16S rDNA gene sequencing and untargeted metabolomics revealed that HQBHG reversed CPT-11-induced disordered amino acid metabolism of phenylalanine, tyrosine, tryptophan and glycine, which was closely related to the diversity and abundance of gut microbiota such as Escherichia-Shigella and Clostridium_sensu_stricto. Therefore, HQBHG has the potential to be an effective agent for the treatment of CIM by inhibiting the NF-kappa B pathway, activating the Nrf2/Keap1 pathway and regulating the amino acid metabolism balance in the gut.