文献类型：期刊文献

中文题名：芍药苷改善睡眠作用机制研究

英文题名：Study on sleep improving mechanism of Paeoniflorin

作者：李越峰[1];徐富菊[1];张泽国[1];李玉萍[2];严兴科[1]

第一作者：李越峰

机构：[1]甘肃中医学院药学院;[2]黑龙江中医药大学附属医院

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2015

卷号：31

期号：1

起止页码：56

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基金资助项目(81460611);教育部科学技术研究重点基金资助项目(212186);甘肃省自然科学基金资助项目(2010:1010RJZA212;2014:145RJZA076);甘肃省财政厅高校基本科研业务费专项基金资金项目(2013-2);甘肃中医学院引进人才科研启动基金资助项目(2010-3)

语种：中文

中文关键词：四逆散冻干粉;芍药苷;改善睡眠;机制

外文关键词：Si Ni San Freeze-dry Powder; Paeoniflorin; improving sleeping; mechanism

摘要：目的研究四逆散冻干粉主要化学成分芍药苷改善睡眠的作用机制。方法取Wistar大鼠平分为4组(每组10只):正常组、四逆散冻干粉组、白芍冻干粉组、芍药苷组。称取冻干粉20 g,加水溶解配成0.5 kg·L-1溶液,灌胃给药,正常组给予等体积蒸馏水。每天1次,连续7 d。用HPLC方法测定大鼠血清和脑脊液中移行成分。结果给予四逆散冻干粉主要化学成分芍药苷后,脑脊液中内源性物质峰面积约是空白脑脊液峰面积的12.5倍,表明芍药苷能明显促进脑脊液中内源性物质的分泌,从而达到改善睡眠作用,该内源性物质最终确定为5-羟色胺。结论四逆散冻干粉主要化学成分芍药苷是通过促进脑脊液中内源性物质5-羟色胺的分泌达到改善睡眠作用。
Objective To study on sleep improving mechanism of Si Ni San Freeze- dry Powder.Methods Wistar rats were divided into four groups,each group was 10.Which were normal group,Si Ni San powder group,Radix Paeoniae Alba group.Paeoniflorin group.The freeze- dried powder 20 g,water dissolved into 0.5 kg · L^-1 solution,administered orally,the normal group were given equal volume of distilled water,1 times a day,continuous 7 d.Blood transitional component were analzed by HPLC method.Results By the analysis of the compound in cerebrospinal fluid,the endogenous substance in cerebrospinal fluid was increased obviously.Increasing peak area of the endogenous substance in cerebrospinal fluid were about 12.5 times the blank＇s in cerebrospinal fluid.Conclusion Paeoniflorin in Si Ni San Freeze- dry Powder improves sleep by promoting the secretion of 5- serotonin in cerebrospinal fluid.