文献类型：期刊文献

英文题名：Glutathione-modified graphene quantum dots as fluorescent probes for detecting organophosphorus pesticide residues in Radix Angelica Sinensis

作者：Mu, Xi-Qiong[1];Wang, Dan[1];Meng, Ling-Yu[1];Wang, Yin-Quan[1,2,3];Chen, Juan[4]

第一作者：Mu, Xi-Qiong

通信作者：Wang, YQ[1];Chen, J[2]

机构：[1]Gansu Univ Chinese Med, Sch Pharm, Lanzhou 730101, Peoples R China;[2]Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou 730000, Peoples R China;[3]MOE PRC, Lanzhou 730000, Peoples R China;[4]Lanzhou Univ, Sch Pharm, Lanzhou 730101, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Pharm, Lanzhou 730101, Peoples R China;[2]corresponding author), Lanzhou Univ, Sch Pharm, Lanzhou 730101, Peoples R China.|[10735]甘肃中医药大学;

年份：2023

卷号：286

外文期刊名：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

收录：;EI(收录号：20224313003817);Scopus(收录号：2-s2.0-85140337214);WOS:【SCI-EXPANDED(收录号:WOS:000878637500005)】；

基金：This work was supported by Strategic Research and Consulting Project of Chinese Academy of Engineering (GS2021ZDA06), Double first-class major scientific research of Gansu Provincial Department of Education (GSSYLXM-05), Special program of poverty alleviation science and technology of Gansu University of Chinese Medicine (2019FPZX-1) and Open Found of Northwest Collaborative Innovation Center for Traditional Chinese Medicine Co-constructed by Gansu Province & MOE of PRC (Xbzzy-2022-01). The funders have no role in the design of the study and collection, analysis, and interpretation of data, or preparation of the manuscript.

语种：英文

外文关键词：Glutathione; Graphene quantum dots; Organophosphorus pesticides residues; Fluorescence probe; Radix Angelica Sinensis

摘要：A novel fluorescent sensor was developed in this study based on glutathione-functionalized graphene quantum dots (GQDs@GSH) to detect organophosphorus pesticide residues in Radix Angelica Sinensis. GQDs@GSH was synthesized by a one-step pyrolysis method with a fluorescence quantum yield as high as 33.9% and its structure was characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. GQDs@GSH exhibited excellent fluorescence property showing strong blue fluorescence under UV irradiation. The fluores-cence of GQDs@GSH could be quenched by Fe3+ by electron transfer and the quenched fluorescence could be recovered due to the strong chelating and reducing ability of phytic acid (PA). Under the catalyzation of acetylcholinesterase (AChE) and choline oxidase (ChOx), acetylcholine (ACh) could be decomposed to H2O2, which could further oxidize Fe2+ to Fe3+ thus quenching the fluorescence of GQDs@GSH once again. Couma-phos, a kind of organophosphorus pesticide, could inhibit AChE activity, thus making the quenched fluorescence turn on again. Several parameters influencing the fluorescence response such as Fe3+, PA, ACh and coumaphos concentration, pH value and reaction time were optimized. Based on such a fluorescence "off-on-off-on" ngkmechanism, GQDs@GSH was successfully applied to the detection of coumaphos in Radix Angelica Sinensis. A good linear relationship between the fluorescence intensity and coumaphos concentration was obtained in the range of 0.1-10.0 mu mol.L- 1. By a standard addition method, the recoveries were measured to be 101.44-117.90% with RSDs lower than 1.98%. The biosensor system is simple, sensitive and accurate. It has a good application prospect in the detection of organophosphorus pesticide residues in traditional Chinese medicine and agricultural products, and also expanded the application scope for glutathione as a highly selective biological molecule.