文献类型：期刊文献

中文题名：健步通络熏蒸液对KOA大鼠血清和关节液中TNF-α、MMP-3的影响

英文题名：The Fluid of JianbuTongluo Fumigation on TNF-α and MMP-3 of Serum and Synovial Fluid in Rats with Knee Osteoarthritis

作者：王钢[1];田杰祥[2];王佳[1];王涛[1]

第一作者：王钢

机构：[1]甘肃中医学院附属医院,兰州730000;[2]甘肃中医学院

第一机构：甘肃中医药大学第二附属医院

年份：2011

卷号：19

期号：12

起止页码：7

中文期刊名：中国中医骨伤科杂志

外文期刊名：Chinese Journal of Traditional Medical Traumatology & Orthopedics

收录：CSTPCD

基金：甘肃省科技厅(项目编号:1010RJZA131)

语种：中文

中文关键词：关节炎;健步通络熏蒸液;细胞因子;软骨

外文关键词：Osteoarthritis; Fluid of JianbuTongluo fumigation; Cytokincs; Cartilage

摘要：目的:探索健步通络熏蒸液防治膝关节骨性关节炎的机制及其临床意义,为辨证施治提供依据。方法:选用Wistar大鼠32只,随机分为正常组,健步通络熏蒸液组,模型组,扶他林组。建立膝关节骨性关节炎模型,用药8周后处死动物,酶联免疫吸附法检测血清和关节液中IL-1β、MMP-3的含量及行病理切片观察软骨组织的形态学变化。结果:膝骨性关节炎大鼠血清和关节液中TNF-α、MMP-3明显高于正常组和治疗组。结论:健步通络熏蒸液减少了各种细胞活性因子的渗出,从而减轻了各种细胞因子对关节软骨的损害,直接或间接改善了软骨基质代谢,促进软骨修复。
Objective：To explore the mechanism and clinical significant of the fluid of JianbuTongluo fumigation for conrolling and ma ring knee osteoarthritis, and to provide the basis for the syndrome differentiation. Methods：Thirty-two Wistar rats were randomty di vided into normal group, JianbuTongluo group, model group, and Voltaren group. The model of osteoarthritis knee was establishment and the rats were killed 8 weeks after treatment. The levels of TNFα and MMP3 in serum and synovial were detect by ELSA method and the morphologieal changes of cartilage was observed by pathological examination. Results：The levels of TNF-α and MMP 3 in rats serum and synovial of model group were obviously higher than the normal group and treatment group. Conclusion.. The flukl of jianbu Tongluo fumigation can improve the cartilage matrix metabolism and promote repaired of cartilage by reducing the leakage of cell activity factor and thereby reducing articular cartilage damage by variety cytokines.