文献类型：期刊文献

中文题名：运脾通肠方提取工艺优化及挥发油成分GC-MS解析

英文题名：Optimization of extraction process of Yunpi Tongchang prescription and GC-MS analysis of volatile oil components

作者：张兆芳[1,2];燕玉奎[1,2];李莉霞[3];宋鹏[1,2];辛二旦[1,2];张晓燕[4];宋治荣[1,2];王耀鹏[1,2]

第一作者：张兆芳

机构：[1]甘肃中医药大学附属医院,甘肃兰州730020;[2]甘肃省中药炮制及质控工程技术研究中心,甘肃兰州730000;[3]甘肃中医药大学,甘肃兰州730000;[4]武威市中医医院,甘肃武威733000

第一机构：甘肃中医药大学第二附属医院

年份：2025

卷号：44

期号：6

起止页码：23

中文期刊名：中兽医医药杂志

外文期刊名：Journal of Traditional Chinese Veterinary Medicine

基金：甘肃省中医药管理局科研项目(GZKP-2020-32)。

语种：中文

中文关键词：运脾通肠方;挥发油;正交试验法;GC-MS;成分解析

外文关键词：Yunpi Tongchang prescription;volatile oil;orthogonal test;GC-MS;component analysis

摘要：优化运脾通肠方的提取工艺并解析挥发油成分。采用超声提取法,在单因素考察基础上,结合正交试验法优化运脾通肠方的提取工艺,采用水蒸气蒸馏法测定挥发油得率并对其成分进行GC-MS解析。结果显示,优选的最佳提取工艺为A_(2)B_(3)C_(3),即无水乙醇超声提取,液料比为22∶1(mL∶g),提取温度60℃,提取次数3次;挥发油得率为0.29%,原药材中挥发油得率为0.35%,共鉴定出85种挥发性成分,主要包括酸类、酯类、醛类、烷烃类等。超声结合有机溶剂提取中药复方挥发油,操作简单、工艺稳定,可为复方化学成分的研究提供参考。
The objective was to optimize the extraction process of Yunpi Tongchang prescription and analyze the components of volatile oil.On the basis of single factor investigation,ultrasonic extraction method was used combined with orthogonal test to optimize the extraction process of Yunpi Tongchang prescription and steam distillation method was used to determine the yield of volatile oil and its components were analyzed by GC-MS.The results showed that the optimal extraction process was A_(2)B_(3)C_(3),ultrasonic extraction with anhydrous ethanol and the ratio of liquid to solid was 22∶1(mL∶g),the extraction temperature was 60℃,and the number of extractions was 3 times.The yield of volatile oil was 0.29% and the yield of volatile oil in the original medicinal plants was 0.35%.A total of 85 volatile components were identified,mainly including acids,esters,aldehydes,alkanes,etc.The extraction of volatile oil from traditional Chinese medicine compound by ultrasound combined with organic solvents is simple to operate and technically stable,which can provide a reference for the study of chemical components of traditional Chinese medicine compound.