文献类型：期刊文献

中文题名：高效液相色谱法测定当归芍药散不同极性部位中阿魏酸和芍药苷的含量

英文题名：Content determination of ferulic acid and paeoniflorin of different polar parts in Danggui Shaoyao San(当归芍药散) by HPLC

作者：刘雪枫[1,2];梁希晨[2];岳栋芳[2];钱倩[1,3];刘东玲[1]

第一作者：刘雪枫

机构：[1]甘肃省中药药理与毒理重点实验室,甘肃兰州730000;[2]甘肃中医药大学药学院,甘肃兰州730000;[3]甘肃省人民医院药剂科,甘肃兰州730000

第一机构：甘肃省中药药理与毒理重点实验室,甘肃兰州730000

年份：2019

卷号：36

期号：1

起止页码：33

中文期刊名：甘肃中医药大学学报

外文期刊名：Journal of Gansu University of Chinese Medicine

基金：甘肃省中药药理与毒理重点实验室开放基金项目(ZDSYS-KJ-2015-004);甘肃省中药药理与毒理重点实验室自主基金项目(ZDSYS-ZZ-2015-003);甘肃中医药大学中青年基金项目(2305016601)

语种：中文

中文关键词：当归芍药散;阿魏酸;芍药苷;高效液相色谱法;含量测定

外文关键词：Danggui Shaoyao San(当归芍药散);ferulic acid;paeoniflorin;HPLC;content determination

摘要：目的建立当归芍药散不同极性部位中阿魏酸和芍药苷的含量测定方法。方法采用高效液相色谱(HPLC)法测定当归芍药散不同极性部位中阿魏酸和芍药苷的含量,色谱柱为Kromasil 100-5-C18柱(4.6 mm×250.0 mm),流动相为甲醇∶0.2%磷酸溶液,梯度洗脱,流速1 mL/min,阿魏酸、芍药苷的检测波长分别为320,230 nm,进样量10μL,柱温30℃。结果阿魏酸和芍药苷的回归方程分别为Y=3.53×10~4X-4.83×10~2(r=0.999 0),Y=1.50×10~4X-2.51×10~4(r=0.999 9),线性范围分别为6.076～303.800μg/mL和7.448～372.400μg/mL;方法学考察中精密度、稳定性和重复性均符合要求,加样回收率的RSD分别为1.2%和1.1%。含量测定结果显示,除醇提物的水相部分未检出阿魏酸外,其他部分中均存在阿魏酸和芍药苷,且水煎液中阿魏酸、芍药苷含量均最高。结论该法简便、准确、分离效果好,可用于当归芍药散不同极性部位中阿魏酸、芍药苷的含量测定。
Objective To establish a content determination method for ferulic acid and paeoniflorin of different polar parts in Danggui Shaoyao San(当归芍药散).Methods HPLC was used with Kromasil 100-5-C18 column(4.6 mm×250.0 mm).The mobile phase was methanol ∶ 0.2% phosphoric acid solution with gradient elution.The flow rate was 1.0 mL/min,the detection wavelength of ferulic acid and paeoniflorin was 320 nm and 230 nm,respectively.The column temperature was 30 ℃,and sample size was 10 μL.Results The regression equations of ferulic acid and paeoniflorin were Y = 3.53×10~4 X-4.83×10~2(r=0.999 0) and Y=1.50×10~4 X-2.51×10~4(r=0.999 9),respectively.The linearity ranged from 6.076 to 303.800 μg/mL for ferulic acid,and from 7.448 to 372.400 μg/mL for paeoniflorin.The precision,stability and repeatability of methodological investigation meet the requirements.The RSD of sample recovery rate was 1.2% and 1.1%,respectively.The results of content determination showed that ferulic acid and paeoniflorin existed in other parts except in the aqueous phase of methanol extract,and the content of ferulic acid and paeoniflorin in water decoction was the highest.Conclusion The method is simple and accurate,with good separating effect.It can be used for content determination of ferulic acid and paeoniflorin of different polar parts in Danggui Shaoyao San.