文献类型：期刊文献

英文题名：Light-Induced Flavonoid Biosynthesis in Sinopodophyllum hexandrum with High-Altitude Adaptation

作者：Zhao, Qiaozhu[1];Dong, Miaoyin[1];Li, Mengfei[1,2];Jin, Ling[3];Pare, Paul W.[4]

第一作者：Zhao, Qiaozhu

通信作者：Li, MF[1];Li, MF[2];Jin, L[3];Pare, PW[4]

机构：[1]Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Peoples R China;[2]Gansu Agr Univ, State Key Lab Arid Land Crop Sci, Lanzhou 730070, Peoples R China;[3]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730101, Peoples R China;[4]Texas Tech Univ, Dept Chem & Biochem, Lubbock, TX 79409 USA

第一机构：Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Peoples R China

通信机构：[1]corresponding author), Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Peoples R China;[2]corresponding author), Gansu Agr Univ, State Key Lab Arid Land Crop Sci, Lanzhou 730070, Peoples R China;[3]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730101, Peoples R China;[4]corresponding author), Texas Tech Univ, Dept Chem & Biochem, Lubbock, TX 79409 USA.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2023

卷号：12

期号：3

外文期刊名：PLANTS-BASEL

收录：;Scopus(收录号：2-s2.0-85147862088);WOS:【SCI-EXPANDED(收录号:WOS:000929580100001)】；

基金：This research was funded by the State Key Laboratory of Aridland Crop Science Gansu Agricultural University (GSCS-2021-Z03), Assurance Project of Ecological Planting and Quality of Daodi Herbs (202103003), and National Natural Science Foundation of China (81560617).

语种：英文

外文关键词：Sinopodophyllum hexandrum; high altitude; flavonoid biosynthesis; gene expression; high light

摘要：Sinopodophyllum hexandrum is a perennial alpine herb producing the anti-cancer metabolite podophyllotoxin (PPT). Although the adaptation of S. hexandrum to high altitudes has been demonstrated and the effects of temperature, precipitation, and UV-B light on plant growth and metabolite accumulation have been studied, knowledge on the role of flavonoid biosynthesis in adapting to high altitudes is limited. In this study, light intensity, amount and type of flavonoids, and differentially expressed proteins (DEPs) and genes (DEGs) at 2300 and 3300 m were analyzed by HPLC, proteomic, transcriptomic, and qRT-PCR analysis. We found that higher light intensity correlated with greater flavonoid, flavonol, and anthocyanin content as well as higher anthocyanin to total flavonoid and flavonol ratios observed at the higher altitude. Based on proteomic and transcriptomic analyses, nine DEPs and 41 DEGs were identified to be involved in flavonoid biosynthesis and light response at 3300 m. The relative expression of nine genes (PAL, CHS1, IFRL, ANS, MYB4, BHLH137, CYP6, PPO1, and ABCB19) involved in flavonoid biosynthesis and seven genes (HSP18.1, HSP70, UBC4, ERF5, ERF9, APX3, and EX2) involved in light stress were observed to be up-regulated at 3300 m compared with 2300 m. These findings indicate that light intensity may play a regulatory role in enhancing flavonoid accumulation that allows S. hexandrum to adapt to elevated-altitude coupled with high light intensity.