文献类型：期刊文献

中文题名：桔梗雄性不育花蕾抗氧化及渗透调节物代谢特性研究

英文题名：Antioxidant and osmoregulation substance properties of flower buds in Platycodon grandiflorus male sterile lines

作者：刘自刚[1];杨亚丽[1];呼天明[2];汪玉琳[1];许瑞[3];雷燕妮[1];何军[1]

第一作者：刘自刚

机构：[1]商洛学院生物医药工程系,陕西商洛726000;[2]西北农林科技大学动物科学学院,陕西杨凌712100;[3]甘肃中医学院护理系,甘肃兰州730000

第一机构：商洛学院生物医药工程系,陕西商洛726000

年份：2012

卷号：21

期号：2

起止页码：77

中文期刊名：草业学报

外文期刊名：Acta Prataculturae Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：陕西省科学技术发展计划项目(2008k16-02);陕西省教育厅专项科研计划项目(2010JK525);甘肃省中医药科研立项课题(GZK-2010-41)资助

语种：中文

中文关键词：桔梗;雄性不育;活性氧;物质代谢

外文关键词：Platycodon grandiflorus; male sterility reactive oxygen species substance metabolism

摘要：在桔梗花器官发育过程中,取不同发育时期的桔梗花蕾,测定其可溶性糖含量、游离脯氨酸含量、可溶性蛋白质含量、超氧化物歧化酶活性、过氧化物酶活性、过氧化氢酶活性和丙二醛含量,比较分析桔梗不育材料与可育材料花蕾发育过程中生理生化特性差异。结果表明,1)花前花蕾发育的各阶段,桔梗不育花蕾可溶性糖含量、游离脯氨酸含量和可溶性蛋白质含量均低于相应可育花蕾,其中尤以游离脯氨酸表现最为明显,随开花前花蕾的发育,可育花游离脯氨酸含量不断升高,而不育花蕾中其含量较低且变化很小。2)与可育花蕾相比,不育花蕾在发育过程中SOD、POD、CAT活性变化趋势基本相同,但其活性明显高于可育花蕾。3)在桔梗花蕾发育的各阶段,不育花丙二醛含量均明显高于相应可育花。桔梗不育花蕾(开花前)生长发育过程中,体内活性氧代谢紊乱、丙二醛积累及游离脯氨酸等"物质代谢损亏"可能是引起桔梗雄性败育的原因。
Male sterility is very important in the utilization of hybrid heterosis in Platycodon grandiflorus. The physiological and biochemical mechanisms of male sterile material, the contents of soluble sugar, free proline, soluble protein, and the activity of superoxide dismultase （SOD）, peroxidase （POD）, and catalase （CAT） in flower buds at different developmental stages of male sterile lines and viable lines were investigated. The re- suits showed that： 1） The contents of soluble sugar, free proline, and soluble protein in flower buds at differ- ent developmental stages of male sterile lines was lower than those of viable lines. Free proline irreversibly in- creased in male sterile buds as flower buds developed, but its content in viable buds was lower. 2） The varia- tion in activity of reactive oxygen species and scavenger enzymes was almost same between sterile buds and via- ble buds during bud development, whereas activities of SOD, POD, and CAT was higher in male sterile buds than in viable buds. 3） The malondialdehyde （MDA） content of male sterile buds was higher than that of viable buds at all times. High activity of reactive oxygen species, scavenger enzymes, high content of MDA and low content of free proline may be the results of male sterility in P. grandiflorus.