文献类型：期刊文献

中文题名：基于16S rDNA序列分析骨质疏松大鼠肠道菌群结构变化及固本增骨方的调控机制

英文题名：Analysis of intestinal flora structure changes and regulatory mechanism of Guben Zenggu Formula in osteoporotic rat model based on 16S rDNA sequence

作者：巩彦龙[1];董万涛[2];宋敏[1];董平[1];黄凯[1];文浩楠[1];海云翔[1]

第一作者：巩彦龙

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃中医药大学附属医院,兰州730000

第一机构：甘肃中医药大学

年份：2019

卷号：34

期号：5

起止页码：2040

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：甘肃省自然科学基金计划项目(No.17JR5RA056;No.1506RJ ZA048);甘肃省中药现代制药工程研究院项目(N o.Y W W-2015049);甘肃省中医药防治慢性疾病重点实验室开放基金项目(No.GSMBKY2015-07);甘肃省中医药管理局科研课题(No.GZK-2016-17)~~

语种：中文

中文关键词：16S;rDNA序列分析;骨质疏松;肠道菌群;固本增骨方

外文关键词：16S rDNA sequence analysis;Osteoporosis;Intestinal flora;Guben Zenggu Formula

摘要：目的:通过16S rDNA序列分析探讨骨质疏松模型大鼠肠道菌群结构的多样性特征及固本增骨方作用的靶点菌种。方法:将48只Wistar雌性大鼠,随机分为造模组(40只)和空白对照组(8只),造模组采用去卵巢法复制骨质疏松模型,造模成功后随即分为模型对照组、戊酸雌二醇组、固本增骨方(高、中、低剂量)组,每组8只。固本增骨方高、中、低剂量组给予固本增骨方(9.2、4.6、2.3g/kg)灌胃,空白对照组和模型对照组以等剂量0.9%氯化钠溶液灌胃,戊酸雌二醇组按90μg/kg给予戊酸雌二醇灌胃,16周后收集大鼠粪便,提取并检测粪菌总DNA,16sRNA扩增子测序,对标本中的细菌16S rRNA V3-V4进行定性分析、OUT分析、16S功能基因预测分析。结果:与空白对照组比较,模型对照组大鼠肠道菌群的多样性增加;与模型对照组比较,戊酸雌二醇组和固本增骨方高、中、低浓度组可调节厚壁菌门、螺旋菌门及Candiadatus Soleaferrea的相对丰度;固本增骨方高剂量组在翻译、细胞壁的生物合成、细胞运动、复制和修复、能源生产与转化等功能方面显著性增高。结论:卵巢切除大鼠诱导骨质疏松模型中,肠道微生态失衡可能为绝经后骨质疏松症的发病机制之一;固本增骨方对肠道菌群具有调节作用,通过"健脾补肾"之效应机制发挥对PMOP的防治作用。
Objective:To analyze the diversity of intestinal flora structure in osteoporosis model rats and the bacterial species of target of Guben Zenggu Formula with the sequence of 16 S rDNA.Methods:Forty-eight female Wistar rats were randomly divided into model group(40 rats)and blank control group(8 rats),the model group was used to replicate the osteoporosis model by ovariectomy,and then divided into model control group,estradiol valerate group,and Guben Zenggu Formula(high,medium and low)group,with 8 in each group.The high,medium and low dose groups of Guben Zenggu Formula were given corresponding concentration by intragastric administration,while the blank control group and the model control group were given isodose normal saline by intragastric administration,the estradiol valerate group was given estradiol valerate with90μg/kg by intragastric administration.After 16 weeks,the feces of rats were collected,and extracted and detected total DNA of the fecal bacteria.The 16 S rRNA amplicon was sequenced,the 16 S rRNA V3-V4 of bacteria in specimens were qualitatively analysis,OUT analysis and 16 S functional genes prediction analysis.Results:Compared with the blank control group,the diversity of rats’intestinal flora increased in the model control group;Compared with the model control group,the estradiol valerate group and the high,medium and low dose groups of the Guben-Zenggu Formula could adjust the relative abundance of phylum firmicutes,spiral phylum and Candiadatus Soleaferrea;In the high dose group of the Guben Zenggu Formula,the function of translation,cell wall biosynthesis,cell movement,replication and repair,energy production and transformation were significantly increased.Conclusion:In the model of osteoporosis induced by ovariectomized rats,the imbalance of intestinal microecology may be one of the pathogenesis of postmenopausal osteoporosis.The Guben Zenggu Formula has the effect of regulating intestinal flora and exerting its preventive and therapeutic effect on PMOP through the effective mechanism of strengthening the spleen and tonifying the kidney.