文献类型：期刊文献

中文题名：基于全基因组甲基化探讨辐射损伤大鼠的旁效应机制

英文题名：Study on the bystander effect mechanism of radiation injury rats based on whole genome methylation

作者：梁建庆[1,2,3];李金田[1,2,4];李娟[3];曾启宇[4];杜雪洋[4];胡蓉[4];张子同[4];李晓伟[4];刘凤艳[5];安耀荣[3]

第一作者：梁建庆

机构：[1]甘肃中医药大学,敦煌医学与转化省部共建教育部重点实验室,兰州730000;[2]甘肃中医药大学博士后流动站,兰州730000;[3]甘肃中医药大学基础医学院,兰州730000;[4]甘肃中医药大学中医临床学院,兰州730000;[5]甘肃中医药大学附属医院,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2018

卷号：33

期号：11

起止页码：5132

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金地区项目(No.81460695,No.81760836);中国博士后科学基金第60批面上资助项目(No.2016M602887)

语种：中文

中文关键词：甲基化;辐射损伤;大鼠;旁效应机制

外文关键词：Methylation;Radiation injury;Rats;Bystander effect mechanism

摘要：目的:从全基因组甲基化的角度探讨辐射损伤大鼠的旁效应发生机制。方法:采用12C6+束照射大鼠右侧肺部构建辐射损伤动物模型,同时设立正常组,取心、肝、脾、左肺、左肾组织以提取DNA。采用MethylRAD技术进行全基因组甲基化检测。结果:与正常组比较,模型组大鼠心、肝、脾、左肺、左肾组织中的甲基化位点(CCGG/CCWGG位点数,CCGG/CCWGG平均深度)显著降低(P<0.01),甲基化位点测序深度显著降低(P<0.01)。两组大鼠基因组不同功能元件上的甲基化位点水平高低不同,差异有统计学意义(P<0.01)。从染色体图谱来看,两组大鼠差异甲基化位点在染色体上的分布是不均匀的。结论:全基因组甲基化差异可能是辐射损伤大鼠旁效应的发生机制之一。
Objective： To discuss the mechanism of bystander effect in radiation injured rats from the point of whole genome methylation. Methods： Radiation injured rats model was established by 12 C6＋ beam irradiated the right lung of rats. Meanwhile, a normal control group was set up, rats were sacrificed and DNA was extracted from heart, liver, spleen, left lung and left kidney. The whole genome methylation was detected by MethylRAD technique. Results： Compared with the normal group, methylation sites in heart, liver, spleen, left lung, left kidney tissues of model rats（CCGG/CCWGG loci, the average depth of CCGG/CCWGG） decreased significantly（P0.01）, methylation sequencing depth decreased significantly（P0.01）. The different functional components on the genome methylation level in two groups had statistically significant difference（P0.01）. From the chromosome map, the distribution of difference in methylation loci on chromosomes was not uniform in two groups. Conclusion： The difference of whole genome methylation may be one of the mechanisms of the bystander effect of radiation injury rats.