文献类型：期刊文献

中文题名：Affymetrix基因芯片测序技术分析垂体对大鼠椎动脉型颈椎病模型的调控机制

英文题名：Affymetrix gene sequencing technique used to analyze the mechanism of pituitary's regulation of CSA model in rats

作者：宋敏[1];巩彦龙[1];刘涛[1];刘建鸿[1];董万涛[2];刘小钰[1];侯红艳[1]

第一作者：宋敏

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃中医药大学附属医院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2018

卷号：39

期号：2

起止页码：210

中文期刊名：西安交通大学学报（医学版）

外文期刊名：Journal of Xi’an Jiaotong University（Medical Sciences）

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金资助项目(No.81360554);甘肃省中药现代制药工程研究院项目开发资助项目(No.2305083306)~~

语种：中文

中文关键词：基因芯片;垂体;椎动脉型颈椎病(CSA);差异基因;基因表达谱;信号通路

外文关键词：gene chip;pituitary;cervical spondylosis of vertebral artery type(CSA);differential gene;gene expression profile;signaling pathway

摘要：目的分析椎动脉型颈椎病(CSA)模型大鼠垂体组织差异基因表达谱变化,探讨垂体对CSA的调控机制。方法将10只SPF级雄性Wistar大鼠随机分为模型组和正常组,采用复合造模法建立CSA模型,模型成功后取垂体组织,提取总RNA,用全基因芯片检测基因表达谱,筛选差异基因,进行基因本体论(GO)、信号通路分析差异基因在信号通路上的富集程度、分子功能和生物学过程。结果与正常组比较,差异基因表达谱分析显示:差异基因总数为321个(|fold change︱>2,P<0.05),其中表达上调的基因有203个,下调的有118个;富集的GO功能共有1 294条,涉及对细胞间信号转导活性的调节、神经细胞功能的调节、对外界刺激的应激反应及凝血功能的调节、血管形成的调节以及调节内膜系统、细胞周期等;参与调节的信号通路共有145条,包括脂肪细胞因子信号通路、TGF-β信号通路、AMPK信号通路、PPAR信号通路、Wnt信号通路、MAPK信号转导通路等。结论垂体对CSA的调控主要通过对炎性刺激、免疫调节、椎动脉功能及内膜系统等的调节实现。
Objective To analyze differences in gene expression profile of pituitary tissue in cervical spondylosis of vertebral artery type(CSA)model rats and explore the mechanism of adrenal gland's regulation of CSA.Methods Ten SPF male Wistar rats were randomly divided into model group and blank group.The CSA model was established by compound modeling method.The total RNA was extracted from the pituitary;the gene expression profile was detected by whole gene chip,ontology(GO),and signal pathway analysis.ResultsCompared with the normal group,the differential genes' expression profile analysis showed that the total number of the differential genes was 321(fold change︱> 2,P<0.05),with203 up-regulated genes and 118 down-regulated genes.A total of 1 294 genes rich in GO function were involved in the regulation of intercellular signal activity and nerve cell function;the stress response to external stimuli;and the regulation of coagulation function,angiogenesis,endometrial system,and cell cycle;There were 145 signal transducers,including adipocytokine signaling pathway,TGF-βsignal transduction,AMPK signaling pathway,PPAR signal pathway,Wnt signaling pathway,and MAPK signal transduction pathway.Conclusion The pituitary regulates CSA mainly through the inflammatory stimulation,immune regulation,regulation of vertebral artery function,and endometrial system.