文献类型：期刊文献

英文题名：A non-peptide NIR fluorescent probe for detection of chymotrypsin and its imaging application

作者：Mu, Shuai[1,2];Xu, Yixuan[1,2];Zhang, Yintang[3];Guo, Xiumei[1,2];Li, Jian[1,2];Wang, Yali[4];Liu, Xiaoyan[1,2];Zhang, Haixia[1,2]

第一作者：Mu, Shuai

通信作者：Liu, XY[1];Liu, XY[2]

机构：[1]Lanzhou Univ, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China;[2]Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Gansu, Peoples R China;[3]Shangqiu Normal Univ, Coll Chem & Chem Engn, Henan Key Lab Biomol Recognit & Sensing, Shangqiu 476000, Peoples R China;[4]Gansu Univ Chinese Med, Key Lab Tradit Chinese Med Qual & Standard, Lanzhou 730000, Gansu, Peoples R China

第一机构：Lanzhou Univ, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Gansu, Peoples R China.

年份：2019

卷号：7

期号：18

起止页码：2974

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20192006916706);Scopus(收录号：2-s2.0-85065495204);WOS:【SCI-EXPANDED(收录号:WOS:000472921600008)】；

基金：This work was supported by the National Natural Science Foundation of China (NSFC) Fund (No. 21575055), the Fundamental Research Funds for the Central Universities (lzujbky-2017-k09) and the Henan Key Laboratory of Biomolecular Recognition and Sensing (HKLBRSK1802).

语种：英文

外文关键词：Fluorescence - Infrared devices - Substitution reactions

摘要：In this work, a turn-on near-infrared fluorescent probe, hemicyanine derivative (CyB), for the highly selective and sensitive detection of chymotrypsin was developed. The probe is fabricated by incorporating a recognition group of 4-bromobutyryl into the hemicyanine skeleton. Upon the nucleophilic substitution reaction of chymotrypsin and the probe, the probe displayed a remarkable fluorescence enhancement and a visible color change under natural light. This property can be utilized for the detection of chymotrypsin with a detection limit (LOD) of 0.013 U mL(-1). Moreover, the cell and in vivo imaging experiments validated that this probe has the potential ability to detect chymotrypsin in biological systems and can provide a promising application prospect for screening chymotrypsin inhibitors.