文献类型：期刊文献

中文题名：不同盐碱胁迫对红芪种子萌发和幼苗生理特性的影响

英文题名：Effects of different salt-alkaline stress on seed germination and physiological characteristics of Hedysarum polybotrys

作者：张勇[1];韩多红[1];晋玲[2];汪生庆[1]

第一作者：张勇

机构：[1]河西学院农业与生物技术学院;[2]甘肃中医学院

第一机构：河西学院农业与生物技术学院

年份：2012

期号：20

起止页码：3036

中文期刊名：中国中药杂志

外文期刊名：China Journal of Chinese Materia Medica

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家科技支撑计划项目(2007BAI37B01);国家中医药管理局2012年中医药行业科研专项(201207002)

语种：中文

中文关键词：不同盐碱胁迫;红芪;种子萌发;生理特性

外文关键词：Hedysarum polybotrys; salt-alkaline stress; seed germination; physiological characteristics

摘要：目的:通过对不同盐碱胁迫下红芪种子萌发及幼苗生理特性的研究,寻找提高红芪种子及幼苗在不同盐碱胁迫下抗性能力的途径。方法:测定不同盐碱胁迫下红芪种子的发芽势(Gv)、发芽率(Gr)、发芽指数(Gi)和相对盐害率,并对红芪幼苗的叶绿素含量、可溶性蛋白质含量、质膜透性、超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性进行了测定。结果:3种盐碱胁迫处理均引起红芪种子发芽率下降,发芽势、发芽指数降低,同时相对盐害率升高,而且随着盐碱胁迫浓度的增加,抑制作用增强。但对种子的胁迫影响大小为Na2CO3﹥Na2SO4﹥NaCl。随着3种盐碱处理浓度的增大,红芪幼苗中叶绿素含量逐渐较少,可溶性蛋白质的含量也逐渐降低,二者都与盐碱处理浓度呈负相关性,但质膜透性逐渐增大与盐碱处理浓度呈正相关性,SOD,POD活性均不同程度的表现为先上升后下降的趋势,均在Na2SO4和Na2CO3浓度为25 mmol·L-1时出现最大值,而NaCl在浓度为50 mmol·L-1时出现最大值。结论:3种盐碱对红芪种子萌发和幼苗生理指标的抑制效应为Na2CO3﹥Na2SO4﹥NaCl。
Objective： In order to get the method for improving the salt resistance of Hedysarum polybotrys seeds and seed- lings under different salt-alkaline stress, the seed germination and physiological characteristics of 1t. polybotrys seedlings were studied. Method ： Several physiological indexes of H. polybotrys seeds under different salt-alkaline stress, such as the germination vigor, germi- nation rate, relative germination rate, relative salt damage rate were measured. And others indexes of the seedlings like chlorophyll contents, soluble protein contents, the permeability of plasmalemma, the activities of POD and SOD were also measured. Result： Dif- ferent salt-alkaline stress decreased the germination rate, vigor of germinate, germination index, while relative salt damage rate in- creased. With the increased salt-alkaline concentration, the adverse effects became more obvious. The strength of the salts： Na2CO3 〉 Na2SO4 〉 NaC1. With the increase of the salt-alkaline concentration, the chlorophyll contents and the soluble protein contents de- creased, but the permeability of plasmalemma increased. The change trend of SOD and POD activity was similar, it is increased firstly, and then decreased as the stress intensity extended, the most significant increase of Na2 SO4 and Na2 CO3 in the concentration of salt-al- kaline was 25 mmol · L-1, but NaC1 was 50 mmol · L-1. Conclusion： The seeds and seedlings inhibition of the salts was Na2 CO3 〉 Na2SO4 〉 NaCl.