文献类型：期刊文献

中文题名：基于补脾胃泻阴火理论指导下莪连颗粒干预无蒂锯齿状病变的研究思路

英文题名：Research ideas on the intervention of E’lian Granule(莪连颗粒)in sessile serrated lesion based on the theory of tonifying the spleen-stomach and reducing yin fire

作者：余金钟[1];毛兰芳[2];凌江红[1];李甫[1];张训兵[1]

第一作者：余金钟

机构：[1]上海中医药大学附属曙光医院,上海201203;[2]甘肃中医药大学附属医院,甘肃兰州730020

第一机构：上海中医药大学附属曙光医院,上海201203

年份：2025

卷号：36

期号：9

起止页码：1700

中文期刊名：时珍国医国药

外文期刊名：Lishizhen Medicine and Materia Medica Research

收录：;北大核心:【北大核心2023】；

基金：国家中医脾胃病优势专科专项经费开放基金项目(2023PW-03/06);兰州市科技发展计划项目(2023-2D-200)。

语种：中文

中文关键词：莪连颗粒;无蒂锯齿状病变;G1阻滞;Skp2/p27

外文关键词：E'lianGranules(莪连颗粒,ELG);Sessile serrated lesion(SSL);Gl arrest;Skp2/p27

摘要：锯齿状途径是无蒂锯齿状病变(SSL)发展为结直肠癌的重要通路,Skp2/p27信号通路诱导G1阻滞是阻断锯齿状途径抑制SSL发展的关键。莪连颗粒受蔡淦学术思想启迪研制而成,前期研究证实其治疗脾虚瘀热型慢性萎缩性胃炎(CAG)及其癌前病变临床疗效显著。基于异病同治的重要治则,脾虚瘀热证是SSL和CAG共同的病机。基于上述的研究,提出科学假说:莪连颗粒可能通过Skp2/p27信号通路诱导G1阻滞阻断锯齿状途径抑制SSL的发展。为验证上述科学假说,该研究拟采用SSL-PDO原位移植方式建立SSL小鼠类器官模型,应用细胞培养技术建立SSL-PDO类器官细胞模型,采用现代技术和方法,通过体内外研究明确莪连颗粒通过Skp2/p27信号通路诱导G1阻滞阻断“锯齿状途径”抑制SSL发展的作用机制,为补脾胃泻阴火学术思想指导下中医治则治法干预SSL提供思路和方法,是中医学治则的具体体现。
The serrated pathway is a critical route through which sessile serrated lesion(SSL)progress to colorectal cancer.The Skp2/p27 signaling pathway-induced G1 arrest plays a pivotal role in blocking the serrated pathway and inhibiting SSL development.Inspired by the academic thoughts of Professor CAI Gan,the formulated E'lian Granule(莪连颗粒,ELG)have demonstrated significant clinical efficacy in treating chronic atrophic gastritis(CAG)characterized by spleen deficiency with blood stasis and heat and its precancerous lesions.Based on the principle of treating different diseases with the same method in traditional Chinese medicine(TCM),spleen deficiency with blood stasis and heat is identified as a shared pathogenesis in both SSL and CAG.Building on previous studies,a scientific hypothesis was proposed that ELG may inhibit SSL progression by blocking the serrated pathway through Skp2/p27 signaling-induced G1 arrest.To validate this hypothesis,this study aims to establish an SSL mouse organoid model using SSL-PDO orthotopic transplantation and develop an SSL-PDO organoid cell model using the cell culture techniques.Utilizing modern technologies and methodologies,both in vivo and in vitro studies will elucidate the action mechanism of ELG inducing G1 arrest through the Skp2/p27 signaling pathway to block the serrated pathway and inhibit the development of SSL.As an embodiment of the principles of TCM treatment strategies,this research provides insights and methods for TCM interventions targeting SSL under the academic thoughts of tonifying the spleen-stomach and draining yin fire.