文献类型：期刊文献

英文题名：Ratiometric Peptide-Based Fluorescent Probe with Large Stokes Shift for Sequential Detection of Hg2+ and S2? and its Applications in Cells Imaging and Smartphone-Assisted Recognition

作者：Wang, Peng[1]; Deng, Weiliang[1]; Li, Shiyang[1]; Zhou, Miao[1]; Zheng, Maoyue[1]; An, Yong[2]

第一作者：Wang, Peng

机构：[1] Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong, 637009, China; [2] The First School of Clinical Medicine, Gansu University of Chinese Medicine, Gansu, Lanzhou, 730030, China

第一机构：Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong, 637009, China

年份：2024

外文期刊名：SSRN

收录：EI(收录号：20240078187)

语种：英文

外文关键词：Amino acids - Cells - Cytology - Fluorescence imaging - Mercury compounds - Smartphones - Titration

摘要：In this work, a simple ratiometric fluorescent probe DKA was synthesized based on the double sides of lysine backbone conjugated with alanine and dansyl groups. DKA exhibited fluorescence ratiometric and "off-on" response for Hg2+ with high sensitivity (19.6 nM), specific selectivity (only Hg2+), strong anti-interference ability (no interference), quick response time (within 60 s) and wide pH range (5-10). The stoichiometry of binding of DKA and Hg2+ was determined to be 1:1 via Job’s plot, ESI-HRMS and 1HNMR titration analysis. Subsequently, the in situ synthesis of DKA-Hg2+ complex was used for highly selective detection of S2? through reversible formation-separation as a novel fluorescence "on-off" probe, and the lowest detection limit for S2? could reach 12.4 nM. In addition, DKA possessed excellent cells permeation and low toxicity, and was used for fluorescence imaging of Hg2+ and S2? in living Hacat cells. Most importantly, the digital imaging using a smartphone color recognition APP indicated that DKA can detect Hg2+ and S2? semi-quantitatively and visually in the field without valuable equipment. ? 2024, The Authors. All rights reserved.