文献类型：期刊文献

中文题名：敦煌固本方对运动负荷大鼠股四头肌相关酶活性及心肌保护作用的研究

英文题名：Myocardial Protection of Dunhuang Guben Recipe and Its Impact on Related Enzyme Activity of Femoral Quadriceps in Exercise Load Rats

作者：李希斌[1];彭雪晶[1];彭志辉[1]

第一作者：李希斌

机构：[1]甘肃中医学院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2014

卷号：46

期号：4

起止页码：193

中文期刊名：新中医

外文期刊名：New Chinese Medicine

基金：甘肃省兰州市城关区科技发展计划项目(编号:BH2012-059);甘肃中医学院中青年基金项目(编号:10-ZQ-03)

语种：中文

中文关键词：敦煌固本方;抗疲劳;心肌保护;动物实验

外文关键词：DunhuangGuben recipe; Anti-fatigue; Myocardial preservation; Animal experiment

摘要：目的:探讨敦煌固本方对运动负荷大鼠股四头肌相关酶活性及对心肌的保护作用。方法:选用健康雄性大鼠60只,饲养1周后。随机分为运动负荷组和非负荷组,每组30只,运动负荷组再随机分为空动组和药动组,每组15只;非运动负荷组再随机分为空静组和药静组,每组15只。苦味酸标记后,药静组和药动组等体积灌胃注射敦煌固本方实验液0.4 mL,空静组和空动组注射等体积0.9%NaCl溶液,连续8周。训练方式为递增负荷游泳训练,持续训练8周。8周后断头处死大鼠,分别取心肌组织样品和股四头肌组织样品,采用比色法检测有关生理生化指标活性。结果:空静组与药动组琥珀酸脱氢酶(SDH)活力比较,差异有显著性意义(P<0.05),药静组与空静组SDH活力比较,差异有非常显著性意义(P<0.01)。药动组与药静组比较苹果酸脱氢酶(MDH)活力、乳酸脱氢酶(LDH)活力比较,差异均有显著性意义(P<0.05)。药动组与药静组丙二醛(MDA)、乳酸(LD)含量和总超氧化物歧化酶(SOD)活力比较,差异均有显著性意义(P<0.05)。结论:敦煌固本方能显著改善运动负荷造成的心肌、骨骼肌缺血缺氧状况。
Objective： To explore the effect of Dunhuang Guben recipe on related enzyme activity of femoral quadriceps and myocardial protection in exercise load rats. Methods： After adaptive feeding for one week, sixty clean rats were randomly divided into exercise load group and non-exercise load group, thirty in each group. The non-exercise load group was then randomized into two subgroups, blank-non-exercise subgroup and drug-non-exercise subgroup, fifteen in each subgroup. The exercise load group was also randomized into two subgroups, blank-exercise subgroup and drug-exercise subgroup, fifteen in each subgroup. After marked by picric acid, rats in the two drug subgroups were orally administered Dunhuang Guben recipe, and rats in the two blank subgroups were given injection of 0.9% NaCI solution, once a day from the first day to the eighth week. All rats in the exercise load group were treated by progressive load swimming training, from the first day to the eighth week. On the 8th week, all rats were sacrificed, and myocardial tissue and femoral quadriceps were sampled for the detection of physiobiochemic indexes by colorimetry. Results： The difference of succinodehydrogenase （SDH） between blank-non-exercise subgroup and drug-exercise subgroup was significant （P 〈 0.05）, and the difference between drug-non-exercise subgroup and blank-non-exercise subgroup was markedly significant （P 〈 0.01）. Significant differences of malate dehydrogenase（MDH）, lactic dehydrogenase（LDH） , and malondialdehyde（MDA） , LD, superoxide dismutase（SOD） were also found between drug-non-exercise subgroup and drug-exercise subgroup（P 〈 0.05）. Conclusion： Dunhuang Guben recipe can significantly improve hypoxic-ischemic state of cardiac muscle and skeleton muscle caused by exercise load.