文献类型：期刊文献

中文题名：藏药二十五味儿茶凝胶对慢性湿疹模型大鼠血清中CCL17及CCL18表达的影响

英文题名：Effect of Tibetan Medicinal 25 Flavor Catechu Gel on Serum Expressions of CCL17 and CCL18 in Rats with Chronic Eczema

作者：费晓影[1];王思农[1];王亚红[1];李丹[1]

第一作者：费晓影

机构：[1]甘肃中医药大学,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2021

卷号：38

期号：12

起止页码：31

中文期刊名：中医药信息

外文期刊名：Information on Traditional Chinese Medicine

基金：西北中藏药协同中心重点项目(XBXT2015-3)。

语种：中文

中文关键词：慢性湿疹;藏药二十五味儿茶凝胶;CCL17;CCL18

外文关键词：Chronic eczema;Tibetan medicinal 25 catechu gel;CCL17;CCL18

摘要：目的:探讨藏药二十五味儿茶凝胶对慢性湿疹大鼠血清中CCL17及CCL18表达的影响。方法:将60只SD大鼠随机分为空白组、模型组、阳性药物组(对照组)、藏药二十五味儿茶凝胶低剂量组(低剂量组)、藏药二十五味儿茶凝胶中剂量组(中剂量组)和藏药二十五味儿茶凝胶高剂量组(高剂量组),每组10只。除空白组外,其余各组大鼠采用2,4-二硝基氯苯(DNCB)诱导的方式制备背部慢性湿疹模型,造模成功后开始干预。对照组涂抹青鹏软膏0.02 g/(cm^(2)·d),模型组涂抹凡士林0.02 g/(cm^(2)·d),低、中、高剂量组分别涂抹0.0025、0.005、0.01 g/(cm^(2)·d)剂量的藏药二十五味儿茶凝胶。肉眼观察大鼠背部慢性湿疹皮损程度并进行评分,采用ELISA法检测大鼠血清中趋化因子CCL17和CCL18水平。结果:藏药二十五味儿茶凝胶各剂量组和对照组均能够有效减轻大鼠背部慢性湿疹症状,降低皮损程度评分(P<0.05),降低血清CCL17、CCL18水平(P<0.05),藏药二十五味儿茶凝胶各剂量组呈剂量依赖性,其中高剂量组效果最优(P<0.05);与对照组相比,高剂量组大鼠皮损程度、评分及血清CCL17、CCL18水平差异无统计学意义(P>0.05)。结论:藏药二十五味儿茶凝胶能够有效改善湿疹模型大鼠背部皮损症状,可能与下调慢性湿疹大鼠模型趋化因子CCL17及CCL18血清水平有关。
Objective:To investigate the effect of Tibetan medicinal 25 flavor catechu gel on serum expressions of CCL17 and CCL18 in rats with chronic eczema.Methods:60 SD rats were randomly divided into the blank group,the model group,the positive control group,the Tibetan medicinal groups of high-dose,medium-dose and low-dose,with 10 rats in each group.2,4-dinitrochlorobenzene(DNCB)was used to induce chronic dorsal eczema in rats.The positive control group was given external application of Qingpeng ointment(0.02 g/cm^(2)·d),the model group was treated with Vaseline(0.02 g/cm^(2)·d),and the Tibetan medicinal groups were treated with Tibetan medicinal 25 flavor catechu gel with the dose of 0.0025 g/cm^(2)·d,0.005 g/cm^(2)·d and 0.01 g/cm^(2)·d,respectively.The lesions of chronic eczema on the back of rats were observed by naked eyes and were scored.Serum chemokines of CCL17 and CCL18 were detected by ELISA.Results:The chronic eczema symptoms were significantly alleviated,the scores of skin lesions were remarkably decreased,and the serum levels of CCL17 and CCL18 were greatly reduced in the Tibetan medicinal groups of different doses and in the positive control group(P<0.05);among the Tibetan medicinal groups,the high-dose had the most significant effect.There were no statistical differences in the degree and scores of skin lesion,as well as in the serum levels of CCL17 and CCL18 between the positive control group and the high-dose group(P>0.05).Conclusion:Tibetan medicinal 25 flavor of catechu gel can effectively improve the back lesions of rats with eczema,which may be related to the down-regulation of serum levels of chemokine CCL17 and CCL18 in rats with chronic eczema.