文献类型：期刊文献

中文题名：党参斑枯病病原菌及其生物学特性

英文题名：Pathogen identification and cultural characteristics of leaf spot of Codonopsis pilosula

作者：王艳[1];曾翠云[1];陈红刚[1];朱田田[1];陈秀蓉[2]

第一作者：王艳

机构：[1]甘肃中医药大学;[2]甘肃农业大学草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心

第一机构：甘肃中医药大学

年份：2016

卷号：43

期号：6

起止页码：928

中文期刊名：植物保护学报

外文期刊名：Journal of Plant Protection

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基金(31460013);甘肃省中药材产业科技攻关项目(GYC11-01);甘肃省自然科学基金(1312RJZA92)

语种：中文

中文关键词：党参斑枯病;病原菌鉴定;rDNA-ITS;生物学特性

外文关键词：Codonopsis pilosula leaf-spot; pathogen identification; rDNA-ITS; biological characteristic

摘要：为明确甘肃省党参斑枯病病原菌及其生物学特性,通过形态学和分子生物学方法进行了病原菌的鉴定,同时利用植物病理学常规培养法进行生物学特性的测定,并利用rDNA-ITS序列构建其系统发育树。结果表明：该菌形态特征与党参壳针孢Septoria codonopsidis Ziling相似,分生孢子器球形、近球形,大小为53.8~76.6μm×40.3~62.7μm;分生孢子无色,针形,1~4个隔膜,大小为15.3~38.8μm×0.9~1.2μm。系统发育树进一步显示,引起党参斑枯病的病原菌为党参壳针孢。该菌菌丝生长、分生孢子萌发和产孢的温度范围分别为5~30、5~35、15~25℃,最适温度分别为20~25、20、20℃;连续光照有利于菌丝生长、孢子萌发,连续黑暗有利于病菌产孢;此菌在相对湿度达90%以上时才可萌发,以水中萌发最好;菌丝生长、孢子萌发和产孢的适宜pH范围分别为4.0~10.0、4.51~9.19和7.0~7.5,最适pH分别为4.5、7.0和7.5;党参根浸渍液和葡萄糖液对孢子萌发有较强的促进作用。表明党参壳针孢菌丝生长和孢子萌发的适宜温度偏低,因此该病害在气温偏低及持续阴雨结露条件下发生较重。
Morphological and molecular methods were combined to identify the pathogen of leaf spot of Codonopsis pilosula.Traditional cultural methods of plant pathology were used to test the cultural characteristics and a phylogenic tree was built through rDNA-internal transcribed spacer（ITS）sequencing.Pycnidia were spherical or sub-spherical,53.8-76.6 μm×40.3-62.7 μm in diameter;conidia were hyaline,needle,one to four septate,15.3-38.8 μm×0.9-1.2 μm in diameter.Phylogenic analysis of ITS confirmed that the pathogen was Septoria codonopsidis Ziling.The temperature ranges for hyphal growth,spore germination and production were 5- 30,5-35 and 15- 25℃,respectively,with an optimum temperature of 20- 25,20 and 20℃,correspondingly.Continuous illumination facilitated hyphal growth and spore germination.Continuous darkness benefitted spore production.The pH ranges for hyphal growth,spore germination and production were 4.0- 10.0,4.51-9.19 and 7.0- 7.5,with an optimum of pH 4.5,7.0 and 7.5,respectively.The relative humidity for spore germination was above 90%.Spore germinated well in water.Root extract and dextrose solution stimulated spore germination.The optimum temperature for mycelium growth and spore germination of the pathogen was low,indicating that the disease would be severe under low temperature,wet and continuous rainy weather.