文献类型：期刊文献

英文题名：Complementary Protective Effects of Lactiplantibacillus Plantarum GUANKE and Picolinic Acid Against Influenza A Virus Infection in Mice

作者：Mi, Jielan[1,2];Yue, Kun[2,3];Zhang, Xiao[2,4];Yang, Yuewen[2];Lu, Simin[2,3];He, Yujia[2];Yang, Zhihan[2];He, Siqin[2];Huang, Yuanming[2];Song, Liqiong[2];Ren, Zhihong[2];Xu, Jianguo[1,2,3]

第一作者：Mi, Jielan

通信作者：Ren, ZH[1]

机构：[1]Nankai Univ, Sch Med, Tianjin, Peoples R China;[2]Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Natl Key Lab Intelligent Tracking & Forecasting In, 155,Changbai Rd,Baishan Town, Beijing, Peoples R China;[3]Chinese Acad Med Sci & Peking Union Med Coll, Res Unit Unknown Microbe, Beijing, Peoples R China;[4]Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou, Peoples R China

第一机构：Nankai Univ, Sch Med, Tianjin, Peoples R China

通信机构：[1]corresponding author), Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Natl Key Lab Intelligent Tracking & Forecasting In, 155,Changbai Rd,Baishan Town, Beijing, Peoples R China.

年份：2026

外文期刊名：PROBIOTICS AND ANTIMICROBIAL PROTEINS

收录：;Scopus(收录号：2-s2.0-105033558819);WOS:【SCI-EXPANDED(收录号:WOS:001715649600001)】；

基金：We thank our colleagues and collaborators for their contributions. We are grateful to Dr. Dayan Wang (Chinese Center for Disease Control and Prevention) for providing the mouse-adapted influenza A virus A/Puerto Rico/8/34 (PR8).

语种：英文

外文关键词：Probiotics; Lactiplantibacillus plantarum GUANKE; Influenza A virus; Picolinic acid; Phenyllactic acid; Inflammatory

摘要：Influenza is an acute, highly contagious respiratory infection and remains a major global health threat. Previous studies have shown that the probiotic Lactiplantibacillus plantarum strain GUANKE (GUANKE) alleviates intestinal barrier disruption and lung pathology in influenza A virus (IAV)-infected mice, but its efficacy as a standalone intervention is modest. This study evaluated whether combining GUANKE with picolinic acid (PA) enhances protection through complementary mechanisms. The results showed that oral co-administration of GUANKE and PA provided greater protection than either agent alone in IAV-infected mice. Time-course analyses that PA primarily suppresses viral replication during early infection, whereas GUANKE attenuates inflammation at later stages. Metabolomic profiling and follow-up experiments identified phenyllactic acid is a major mediator of GUANKE's anti-inflammatory activity. In summary, PA limits early viral burden while GUANKE restrains subsequent inflammatory injury, together improving overall protection against IAV in mice. These findings support a probiotic-small-molecule combination strategy that leverages mechanistic complementarity to mitigate influenza.