文献类型：期刊文献

中文题名：刺芒柄花苷对肺癌放疗诱发肠道炎症的影响及机制研究

英文题名：Effects of Ononin on Radiation-induced Intestinal Inflammation in Lung Cancer and Its Underlying Mechanisms

作者：师亮亮[1];陈亚萍[1];李洋洋[1];侯红豆[1];张尚祖[1];陈琰[1];韵成才[1];刘永琦[1,2];魏本君[1,3];张利英[1,2]

第一作者：师亮亮

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃省高校重大疾病分子医学与中医药防治研究重点实验室,兰州730000;[3]甘肃省中医方药挖掘与创新转化重点实验室,兰州730000

第一机构：甘肃中医药大学

年份：2024

卷号：41

期号：22

起止页码：3079

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金项目(82260893、82004094);甘肃省教育科技创新项目(2022B-116);甘肃中医药大学创新创业项目(2023CX02)。

语种：中文

中文关键词：刺芒柄花苷;肺癌;放疗;肠道炎症

外文关键词：ononin;lung cancer;radiotherapy;intestinal inflammation

摘要：目的探究刺芒柄花苷对放疗诱发荷瘤小鼠肠道炎症的作用以及相关作用机制。方法采用C57BL/6小鼠(18~22 g,雄性)40只,随机分为空白组、肿瘤组、辐射组、刺芒柄花苷组。除空白组外,其余小鼠左侧腋窝皮下接种小鼠肺癌Lewis细胞。辐射组和刺芒柄花苷组待肺部肿瘤长到可以触及皮下直径约3~6 mm肿块后进行X射线4 Gy辐照其瘤体2 min,建立肺癌荷瘤辐照小鼠模型。模型制备完成后,次日刺芒柄花苷组予以刺芒柄花苷连续腹腔注射3 d。取材后,苏木素-伊红(HE)染色观察结肠组织病理学形态,ELISA检测IL-33、TNF-α、IL-1β、IL-6含量,免疫组化检测ST2L的表达,免疫荧光检测TRPA1的表达,蛋白免疫印迹法检测ST2L、TRPA1、P-PLCγ、IP3R蛋白的表达。结果与辐射组相比,刺芒柄花苷给药后结肠组织损伤明显改善(P<0.01),肺组织中IL-33水平降低(P<0.05),结肠组织中ST2L、TRPA1、P-PLCγ、IP3R蛋白表达显著下调(P<0.01或P<0.001),结肠组织中炎症因子TNF-α、IL-1β、IL-6水平降低(P<0.01或P<0.001)。结论刺芒柄花苷通过抑制放疗后肺部IL-33的表达进而抑制TRPA1通道的激活,从而缓解肺癌放疗诱发的肠道炎症。
OBJECTIVET To explore the impact of ononin on radiation-induced gastrointestinal inflammation in mice harboring tumors,as well as elucidate the underlying mechanisms.METHODS Forty male C57BL/6 mice(18?22 g)were subjected to random allocation into blank group,tumor group,radiation group,and ononin group.With the exception of the blank group,the remaining mice were subcutaneously administered Lewis lung cancer cells in the left axilla.Once the lung tumors reacheda diameter of approximately 3?6 mm,the radiation group and cynandione A group were subjected to X-ray irradiation at a dosage of 4 Gy for a duration of 2 min.Following successful model establishment,the ononin group received intraperitoneal administrations of ononin for a duration of 3 d.Subsequent to sampling,histopathological morphology of colon tissue was assessed via hematoxylin-eosin(HE)staining.ELISA was employed to determine the levels of IL-33,TNF-α,IL-1β,and IL-6,while immunohistochemistry was employed to track ST2L expression.Notably,immunofluorescence was utilized to monitor TRPA1 expression,and Western blotting was conducted to evaluate the expression levels of ST2L,TRPA1,P-PLCγ,and IP3R proteins.RESULTS Compared with post-irradiation,ononin administration significantly improved colon tissue damage(P<0.01),decreased IL-33 levels in lung tissue(P<0.05),downregulated the expression of ST2L,TRPA1,P-PLCγ,and IP3R proteins(P<0.01 or P<0.001),and reduced the levels of inflammatory factors TNF-α,IL-1β,and IL-6 in the serum(P<0.01 or P<0.001).CONCLUSION Ononin effectively mitigates radiation-induced intestinal inflammation in lung cancer by inhibiting the expression of IL-33,thereby suppressing the subsequent activation of TRPA1 channels.