文献类型：期刊文献

中文题名：当归补血汤含药血清对Ang Ⅱ诱导的心肌细胞凋亡的影响

英文题名：Effect of serum containing Dangguibuxue Decoction on the apoptosis of Angiotesin Ⅱ (Ang Ⅱ)-induced cardiomyocytes

作者：徐厚谦[1];孙艳[1];孙樱丹[1];孙少伯[1]

第一作者：徐厚谦

机构：[1]甘肃中医学院,兰州730000

第一机构：甘肃中医药大学

年份：2013

卷号：29

期号：4

起止页码：16

中文期刊名：中药药理与临床

外文期刊名：Pharmacology and Clinics of Chinese Materia Medica

收录：北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

基金：甘肃省中医药管局(英才计划);编号:GZK-2010-56

语种：中文

中文关键词：当归补血汤;血管经张素Ⅱ;心肌细胞;凋亡

外文关键词：Dangguibuxue Decoction（当归补血汤）;angiotesin Ⅱ;cardiomyocyte;apoptosis

摘要：目的:观察当归补血汤含药血清对AngⅡ诱导的心肌细胞凋亡的影响,探讨其抑制心肌细胞凋亡的机制。方法:将心肌细胞分为4组:空白对照组、AngⅡ(10-7mol/L)组、缬沙坦(10-6mol/L)组、5g/kg当归补血汤1日2次的10%当归补血汤含药血清组。以Annexin V/PI流式细胞仪检测细胞凋亡率,透射电镜观察细胞线粒体超微结构,Western blotting测定凋亡相关蛋白Bax、Bcl-2表达变化。结果:10%当归补血汤含药血清干预后心肌细胞凋亡率明显低于AngⅡ(10-7mol/L)组,心肌细胞Bax蛋白表达显著低于AngⅡ组,Bcl-2蛋白表达显著高于AngⅡ组,Bax/Bcl-2比值显著低于AngⅡ组。结论:10%当归补血汤含药血清对AngⅡ诱导的心肌细胞凋亡有明显的抑制作用,其可能与减少心肌细胞Bax蛋白和增加Bcl-2蛋白表达相关。
Objective ： To study effect of serum containing Dangguibuxue Decoction on the apoptosis of Ang II-induced cardiomyocytes,and discuss its mechanisms.Methods： The cultured cardiomyocytes were divided into 4 groups： control group,angⅡ（10-7mol / L） group,valsartan group（10-6mol / L） and 10% medicated serum group.Apoptosis rate was detected with Annexin V / PI flow cytometric analysis.Mitochondrial injury of the myocardium was studied with the electron microscope.Western Blotting was used to study the change of Bax protein,Bcl-2 protein.Results： The apoptosis rate of 10% medicated serum group was significantly lower than the AngII（10-7mol / L） group（P ＆lt; 0.05）.Bax protein of medicated serum group was obviously lower than the AngII group（P ＆lt; 0.05）.Bcl-2 protein of medicated serum group was significantly higher than the AngII group（P ＆lt; 0.05） Bax / Bcl-2 was significantly lower than the AngII group（P ＆lt; 0.05）.Conclusion： 10% serum containing Dangguibuxue Decoction could inhibit the apoptosis of AngII-induced cardiomyocyte.It may be related with the reduce Bax protein and increase Bcl-2 protein.