文献类型：期刊文献

中文题名：两种诱导子对党参不定根生长及多糖含量的影响

英文题名：Effects of two inducers on the growth and polysaccharide content of adventitious roots of Codonopsis pilosula

作者：王琳佳[1,2,3];徐子杰[1,2,3];张延红[1,2,3];何春雨[1,2,3];郭清毅[1,2,3]

第一作者：王琳佳

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]天然药用种质资源挖掘与新品种选育协同创新中心,甘肃兰州730000;[3]甘肃中医药大学杏林百草园,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2026

卷号：37

期号：11

起止页码：2055

中文期刊名：时珍国医国药

外文期刊名：JOURNAL OF LI-SHIZHEN TRADITIONAL CHINESE MEDICINE

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金(81960683);甘肃省高等学校产业支撑计划项目(2020C-09)。

语种：中文

中文关键词：党参;不定根;诱导子;多糖;抗氧化酶

外文关键词：Codonopsis pilosula;Adventitious roots;Inducers;Polysaccharides;Antioxidant enzymes

摘要：目的明确党参不定根悬浮培养的生长曲线,探究茉莉酸甲酯(MeJa)和壳聚糖(CTS)两种诱导子对不定根生长及多糖含量的影响。方法接种后不同天数测定党参不定根的干鲜重,向不定根悬浮培养体系中分别添加两种不同种类、不同浓度的诱导子。结果党参不定根生长曲线呈“S”型。茉莉酸甲酯的适宜添加浓度为150μmol/L,适宜诱导时间为8天;壳聚糖的适宜添加浓度为50 mg/L,适宜诱导时间为4天。结论在党参不定根的悬浮培养中,可在不定根培养的第15天加入适宜浓度的MeJa或CTS并诱导适宜时间,能显著提高党参不定根的多糖含量。
Objective To clarify the growth curve of the suspension culture of Codonopsis pilosula adventitious roots and to investigate the effects of two inducers,methyl jasmonate(MeJa)and chitosan(CTS),on the growth and polysaccharide content of the adventitious roots.Methods The dry and fresh weights of C.pilosula adventitious roots were measured at different days after inoculation.Two different types of inducers with varying concentrations were added to the adventitious root suspension culture system.Results The growth curve of C.pilosula adventitious roots showed an“S”shape.The optimal concentration of MeJa was 150μmol/L with a suitable induction time of 8 days;the optimal concentration of CTS was 50 mg/L with a suitable induction time of 4 days.Conclusions In the suspension culture of C.pilosula adventitious roots,adding an appropriate concentration of MeJa or CTS on the 15th day of culture and inducing for a suitable duration can significantly increase the polysaccharide content of the adventitious roots.