文献类型：期刊文献

中文题名：外源性L-精氨酸对大鼠背部跨区皮瓣成活的影响

英文题名：Effect of exogenous L-arginie on survival of extended dorsal perforator flaps in rats

作者：李文波[1,2];贾丁丁[1,2];王飞[1];张超[1,2];石杰[1];张洪[1,2];吴路加[3];高秋明[1]

第一作者：李文波

机构：[1]兰州军区兰州总医院全军骨科中心创伤骨科,甘肃兰州730050;[2]甘肃中医药大学研究生院,甘肃兰州730000;[3]南方医科大学研究生院,广东广州510515

第一机构：兰州军区兰州总医院全军骨科中心创伤骨科,甘肃兰州730050

年份：2017

卷号：46

期号：6

起止页码：656

中文期刊名：浙江大学学报（医学版）

外文期刊名：Journal of Zhejiang University(Medical Sciences)

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；PubMed;

基金：甘肃省自然科学基金(1506RJZA298);甘肃省卫生行业科研计划(GSWSKY2017-30)

语种：中文

中文关键词：硝基精氨酸/药理学;外科皮瓣/血液供给;新生血管化;生理性/药物;作用;血管内皮生长因子A;一氧化氮;大鼠;Sprague-Dawley

外文关键词：Nitroarginine/pharmacology ; Surgical flaps/blood supply ;Neovascularization, physiologic/drug effects; Vascular endothelial growth factor A;Nitric oxide ; Rats, sprague-dawley

摘要：目的:观察外源性L-精氨酸对大鼠背部跨区皮瓣成活的影响。方法:雄性SD大鼠16只随机分为L-精氨酸组和对照组,每组8只,分别建立背部三穿支体跨区皮瓣。L-精氨酸组分别于术前1d、术后即刻、术后1～7 d腹腔注射L-精氨酸400 mg·kg^(-1)·d^(-1),对照组于相同时间点腹腔注射等体积等渗氯化钠溶液。术后7 d观察血管走形和分布,计算皮瓣成活率;HE染色观察ChokeⅡ区新生血管并计算新生血管数量和管径;免疫组织化学染色和蛋白质印迹法检测ChokeⅡ区血管内皮生长因子(VEGF)表达。结果:术后7 d,L-精氨酸组有1只大鼠皮瓣全部成活,其余7只在ChokeⅡ区远端出现小面积不同程度坏死;对照组大鼠皮瓣均有不同程度坏死,坏死范围包括邻近ChokeⅡ区和全部ChokeⅡ区以远的皮瓣。术后7 d,两组ChokeⅠ区血管均达到真性吻合,L-精氨酸组ChokeⅡ区新生血管较多,皮瓣末端血管结构较完整,对照组ChokeⅡ区新生血管较少,皮瓣末端发黑坏死,观察不到血管结构。L-精氨酸组皮瓣成活率为(88.42±4.19)%,显著高于对照组(76.52±5.37)%(t=3.707,P<0.01)。术后7 d,L-精氨酸组ChokeⅡ区新生血管数量和管径分别为(29.47±5.28)个/mm^2和(47.27±5.32)μm,明显高于对照组(t=2.694和2.389,P<0.05或P<0.01)。免疫组织化学染色和蛋白质印迹法检测结果显示,L-精氨酸组ChukeⅡ区VEGF表达量明显高于对照组(t=9.428和-3.054,P<0.05或P<0.01)。结论:外源性L-精氨酸可促进大鼠背部跨区皮瓣ChokeⅡ区血管新生和扩张,改善皮瓣血供,提高皮瓣成活率。
Objective： To investigate the effect of exogenous L-Arg on the survival of extended perforator flap in rats. Methods： Sixteen male Sprague Dawley rats were randomly divided into L-Arg group （n = 8） and control group （n = 8 ）. The extended dorsal three-vascular territory perforator flaps were made in rats. L-Arg （400 mg·kg-1· d-1） was injected intraperitoneally in L-Arg group 1 d before operation, immediately and 1 -7 d after operation, while the same volume of saline was injected intraperitoneally in control group at the same time points. The appearance and distribution of blood vessels were observed, and the flap survival areas were measured 7d after operation. The tissue samples were harvested from choke zone Ⅱ for histological study and the expression of vascular endothelial growth factor （VEGF） was detected by immunohistochemistry and Western blot, respectively. Results： After 7d, the clearer vascular structure and more new vessels in choke zone Ⅱ were observed in L-Arg group. The survival rate of flap in L-Arg group was （88.42 ± 4.19）%, which was significantly higher than that in control group [ （76. 52± 5.37） % , t = 3. 707, P 〈 0.01 ]. The microvessel density and caliber of choke zone Ⅱ in L-Arg group was （29.47 ± 5.28 ）/mm2 and （47.27± 5.32）μm, which were significantly higher than those in control group （ t = 2. 694 and 2. 389, P 〈 0.05 or P 〈 0.01 ）. The immunohistochemistry and Western blot showed that the expression of VEGF in choke zoneⅡ of L-Arg group was significantly higher than that in control group （t = 9. 428 and - 3. 054,P 〈 0.05 or P 〈 0.01 ）. Conclusion： Exogenous L-Arg can increase the survival rate of extended dorsal perforator skin flap through promoting vascularization and dilatation of vessels in choke zone Ⅱ in rats.