文献类型：期刊文献

英文题名：The Pro-Tumor Biological Function of IL-36α Plays an Important Role in the Tumor Microenvironment of HCC

作者：Song, Yanmei[1];Chu, Huiyuan[1];Liu, Fang[1];Guo, Wenjie[1];Gao, Na[1];Chen, Che[1];Bao, Shisan[1]

第一作者：宋艳梅

通信作者：Chen, C[1];Bao, SS[1]

机构：[1]Gansu Univ Chinese Med, Dept Clin Lab Diagnost, Sch Publ Hlth, 35 Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学公共卫生学院

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Dept Clin Lab Diagnost, Sch Publ Hlth, 35 Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China.|[10735e9d5e7087247e71b]甘肃中医药大学公共卫生学院;[10735]甘肃中医药大学;

年份：2023

卷号：15

起止页码：895

外文期刊名：CANCER MANAGEMENT AND RESEARCH

收录：;Scopus(收录号：2-s2.0-85170372093);WOS:【SCI-EXPANDED(收录号:WOS:001069642500001)】；

基金：This work was supported by The Natural Science Foundation of Gansu Province (No. 20JR10RA311), The Science and Technology Department Project of Lanzhou District of Gansu Province (No. 2020JSCX0084), and The Postgraduate Innovation Fund, Gansu University of Chinese Medicine (No. 2021CX66). We also acknowledge the staffs from The Second People's Hospital of Lanzhou University and Gansu Provincial People's Hospital for their assistance.

语种：英文

外文关键词：hepatocellular carcinoma; interleukin-36a; prognosis; biomarker

摘要：Purpose: To investigate the role of IL-36 in the tumorigenesis of hepatocellular carcinoma (HCC). IL-36 composed of a natural antagonist (IL-36Ra) and three agonists (IL-36 alpha, -beta, -gamma) that stimulate inflammation by binding to a common receptor consisting of IL-36R and IL-1RAcP. HCC is a common malignancy associated with high morbidity and mortality, often diagnosed at later stages. Although the exact role of IL-36 alpha in HCC remains controversial, it is hypothesized that it may play a significant role in the development and progression of this cancer. Materials and Methods: In the current study, we measured both circulating and intrahepatic levels of IL-36 alpha from HCC patients and healthy controls, using ELISA. The association between IL-36 and the differentiation of HCC was determined. Furthermore, the role IL-36 in both HCC and non-HCC cell lines was evaluated in vitro. Results: Circulating and intra-hepatic IL-36 alpha was inversely correlated with differentiation of HCC, suggesting that IL-36 alpha contribute to protection during the development of HCC. Based on bioinformatics, miR-27b-3p is closely related to downstream IL-36 alpha. Thus, we determined miR-27b-3p expression in HCC tissues, showing upregulated miR-27b-3p was inversely correlated with IL-36 alpha in HCC, perhaps via CXCL1 in HCC cells. It was confirmed that IL-36 alpha inhibited HCC proliferation, viability and migration in vitro, consistent with reduced the expression of cytokines IL-1 beta, IL-18, implying that IL-36 alpha inhibited the possible involvement of pyroptosis. Conclusion: Our data suggests that IL-36 alpha may be a potential therapeutic target and a prediction biomarker for the management of HCC.