文献类型：期刊文献

英文题名：Establishment and optimization of the virus-induced gene silencing (VIGS) system in Angelica sinensis (Oliv.) diels

作者：Guo, Xudong[1];Ji, Jie[1];Yang, Junning[3];Ma, Junfeng[3];Wang, Caixiang[3];Wang, Yuanyuan[1,2];Ma, Xiaohui[1,2];Su, Junji[3];Jin, Ling[1,2]

第一作者：Guo, Xudong

通信作者：Jin, L[1];Su, JJ[2]

机构：[1]Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China;[2]Gansu Pharmaceut Ind Innovat Res Inst, Lanzhou 730000, Peoples R China;[3]Gansu Agr Univ, Coll Life Sci & Technol, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China;[2]corresponding author), Gansu Agr Univ, Coll Life Sci & Technol, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China.|[10735]甘肃中医药大学;

年份：2026

卷号：242

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20261420416792);Scopus(收录号：2-s2.0-105034580346);WOS:【SCI-EXPANDED(收录号:WOS:001705068800001)】；

基金：This study was financially supported by the Joint Research Fund Project of Gansu Province (Grant No. 25JRRA1171) , Research Project on the Protection and Development of Traditional Chinese Medicine Re-sources (Grant No. 2025 4) ,the earmarked fund for the Gansu Agricul-ture Research System (Grant No. GSRAS-06) , the 2026 Gansu Province Graduate Student Innovation Excellence Award (Grant No. 2026CXZX-921) , the China Agriculture Research System of MOF and MARA (Grant No. CARS-21) , the Gansu Province Science and Technology Plan Project: Major Science and Technology Special (Grant No. 23ZDFA013-1) , and the Innovation Center for Traditional Chinese Medicine Co-constructed by Gansu Province & MOE of PRC.

语种：英文

外文关键词：Angelica sinensis (Oliv.) Diels; Virus-induced gene silencing (VIGS); AsPDS; AsCLA1; Tobacco rattle virus (TRV)

摘要：As a renowned traditional medicinal plant in China, Angelica sinensis requires in-depth functional genomic studies to decipher the molecular mechanisms governing its growth and development. Virus-induced gene silencing (VIGS) represents a powerful tool for such investigations owing to its straightforward methodology, short experimental cycle, and capacity for rapid transient gene functional validation. However, the current absence of a robust VIGS system in A. sinensis significantly impedes progress in gene function research. In this study, based on the whole-genome data from A. sinensis, three candidate reporter genes, AsPDS, AsCLA1-1 and AsCLA1-2, were cloned for VIGS vector construction and inserted into a tobacco rattle virus (TRV) silencing vector. Four Agrobacterium-mediated transformation methods, namely, root incision soaking, ultrasound assisted infiltration, vacuum infiltration, and quartz sand abrasion, were evaluated for the silencing of three reporter genes in A. sinensis. The root incision soaking method induced the earliest appearance of the albino phenotype while maintaining a relatively high survival rate. Among the tested genes, AsCLA1-1 demonstrated consistently high silencing efficiency (>50 %) across all methods and was consequently established as the optimal reporter gene. Two-way analysis of variance (ANOVA) revealed a highly significant interaction (P < 0.01) between Agrobacterium concentration (OD600) and cocultivation time. Through multiple statistical comparison methods, the optimal parameters were determined to be an OD600 = 1.60 and 14 h cocultivation. This optimized regimen achieved silencing efficiencies of 62 % at 14 d and 70 % at 28 d, while maintaining excellent plant viability. In summary, this study successfully established and optimized a VIGS system for A. sinensis, providing an effective methodology for investigating the functions of key genes involved in its medicinal compound biosynthesis and growth regulation while also offering a valuable technical framework for establishing VIGS systems in other root and rhizome medicinal plants.