文献类型：期刊文献

中文题名：花椒不同提取方法传导麻醉作用及成分鉴定

英文题名：Study on the effects of conduction anesthesia and component identification of different extraction methods of Zanthoxylum bungeanum

作者：司昕蕾[1,2];边甜甜[1,2];牛江涛[1,2];辛二旦[1,2];曹瑞[1,2];李越峰[1,2]

第一作者：司昕蕾

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃省中药质量与标准研究重点实验室,兰州730000

第一机构：甘肃中医药大学

年份：2020

卷号：35

期号：5

起止页码：2311

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金项目(No.81960713);甘肃省自然科学基金创新基地和人才计划项目(No.18JR3RA197);甘肃省中药质量与标准研究重点实验室开放基金(No.ZYZL18-008)。

语种：中文

中文关键词：花椒/传导麻醉;超高效液相色谱-飞行时间质谱联用技术;离体神经法;黄酮类;酰胺类;香豆素类;酚类化合物

外文关键词：Zanthoxylum bungeanum;Conduction anesthesia;UPLC-QTOF-MS;In vitro neurology;Flavonoids;Amides;Coumarins;Phenols

摘要：目的:研究花椒不同提取方法的传导麻醉作用,并对其进行成分鉴定。方法:以大鼠离体坐骨神经法测定花椒不同提取方法的传导麻醉作用,并以超高效液相色谱-飞行时间质谱联用技术(UPLC-QTOF-MS)法鉴定其化学成分。结果:蒸馏水组大鼠离体坐骨神经神经传导最低电位为(-39.32±1.12)mV,最高电位为(10.86±0.77)mV;盐酸利多卡因组最低电位为(-8.91±1.05)mV,最高电位为(2.38±0.38)mV;0.1g/mL水回流提取液组最低电位为(-22.94±2.88)mV,最高电位为(6.80±0.99)mV;0.2g/mL水回流提取液组最低电位为(-11.78±2.52)mV,最高电位为(3.37±1.01)mV。0.2g/mL花椒水回流提取液与盐酸利多卡因作用类似,均能降低大鼠坐骨神经传导最高电位且升高大鼠坐骨神经传导最低电位。经鉴定,花椒水回流提取液主要含黄酮类、酰胺类、香豆素类及酚类化合物。结论:花椒水回流提取液神经传导麻醉作用类似于盐酸利多卡因,且与羟基-γ-异山椒素、羟基-γ-山椒素、羟基-α-山椒素等化合物相关。
Objective: To study the conduction anesthetic effect of different extraction methods of Zanthoxylum bungeanum and identify its components. Methods: The conduction anesthetic effects of different extraction methods of Zanthoxylum bungeanum were determined by isolated rat sciatic nerve method. The chemical constituents of Zanthoxylum bungeanum were identified by UPLC-TOF-MS method. Results: The lowest potential of isolated sciatic nerve conduction in pure water group was(-39.32±1.12)mV, the highest potential was(10.86±0.77)mV;in lidocaine hydrochloride group, the lowest potential in lidocaine hydrochloride group was(-8.91±1.05)mV, the highest potential was(2.38±0.38)mV;in 0.1 g/mL Zanthoxylum bungeanum water reflux extraction group, the lowest potential was(-22.94±2.88)mV, the highest potential was(6.80±0.99)mV in lidocaine hydrochloride group;in 0.2 g/mL Zanthoxylum bungeanum water reflux extract group, the lowest potential was(-11.78±2.52)mV, and the highest potential was(3.37±1.01)mV. Both Zanthoxylum bungeanum water reflux extract and lidocaine hydrochloride could significantly decrease the highest sciatic nerve conduction potential and increase the lowest sciatic nerve conduction potential in rats. It was identified that the reflux extract of Zanthoxylum bungeanum mainly contained flavonoids, amides, coumarins and phenols. Conclusion: The nerve conduction anesthetic effect of Zanthoxylum bungeanum water reflux extract is similar to that of lidocaine hydrochloride. And it is related to hydroxy-γ-isophorin, hydroxy-γ-capsaicin, hydroxy-α-capsaicin and other compounds.