文献类型：期刊文献

英文题名：Protective Effects and Mechanisms of Huangqi Decoction Against Radiation-Induced Bystander Effects

作者：An, Fangyu[1,2,3,8];Yan, Chunlu[1,2,4];Li, Jiajin[5];Lv, Xueying[5];Wang, Caixia[6];Deng, Jie[7];Shi, Biao[7];Zhang, Jinlong[7];Che, Wenxuan[5];Zheng, Wenrui[5]

第一作者：安方玉;An, Fangyu

通信作者：An, FY[1];An, FY[2];An, FY[3];An, FY[4]

机构：[1]Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis & Study Preve, Lanzhou 730000, Peoples R China;[2]Minist Educ, Key Lab Dunhuang Med, Lanzhou 730000, Peoples R China;[3]Gansu Univ Chinese Med, Lab Biochem, Teaching Expt Training Ctr, Lanzhou 730000, Peoples R China;[4]Gansu Univ Chinese Med, Sch Tradit Chinese & Western Med, Dept Chinese Surg, Lanzhou 730000, Peoples R China;[5]Gansu Univ Chinese Med, Dept Internal Med, Clin Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China;[6]Gansu Univ Chinese Med, Lab Immun, Teaching Expt Training Ctr, Lanzhou 730000, Peoples R China;[7]Gansu Univ Chinese Med, Dept Internal Med, Clin Med Coll 1, Lanzhou 730000, Peoples R China;[8]Gansu Univ Chinese Med, Lab Biochem, Teaching Expt Training Ctr, 35 Dingxi East Rd, Lanzhou 730000, Gansu Province, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis & Study Preve, Lanzhou 730000, Peoples R China;[2]corresponding author), Minist Educ, Key Lab Dunhuang Med, Lanzhou 730000, Peoples R China;[3]corresponding author), Gansu Univ Chinese Med, Lab Biochem, Teaching Expt Training Ctr, Lanzhou 730000, Peoples R China;[4]corresponding author), Gansu Univ Chinese Med, Lab Biochem, Teaching Expt Training Ctr, 35 Dingxi East Rd, Lanzhou 730000, Gansu Province, Peoples R China.|[10735]甘肃中医药大学;

年份：2023

卷号：41

期号：5

起止页码：1527

外文期刊名：INTERNATIONAL JOURNAL OF MORPHOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001145205400016)】；

基金：This work was supported by the National Natural Science Foundation of China (grant no. 81760797) , the Open Fund Project of the Key Laboratory of Dunhuang Medicine, Ministry of Education, China (grant no. DHYX18-15) , and the Improvement Project of College Students'Employment and Entrepreneurship Ability of Gansu Province (grant no. 6-1) .

语种：英文

外文关键词：Huangqi decoction; Inflammatory factors; Endocrine hormone; Apoptosis

摘要：The 12C6+ heavy ion beam irradiation can cause bystander effects. The inflammatory cytokines, endocrine hormones and apoptotic proteins may be involved in 12C6+ irradiation-induced bystander effects. This study characterized the protective effects and mechanisms of Huangqi decoction (HQD) against 12C6+ radiation induced bystander effects. Wistar rats were randomly divided into control, 12C6+ heavy ion irradiation model, and high-dose/medium-dose/low-dose HQD groups. HE staining assessed the pathological changes of brain and kidney. Peripheral blood chemical indicators as well as inflammatory factors and endocrine hormones were detected. Apoptosis was measured with TUNEL. Proliferating cell nuclear antigen (PCNA) expression was determined with real-time PCR and Western blot.Irradiation induced pathological damage to the brain and kidney tissues. After irradiation, the numbers of white blood cells (WBC) and monocyte, and the expression of interleukin (IL)-2, corticotropin-releasing hormone (CRH) and PCNA decreased. The damage was accompanied by increased expression of IL-1 beta , IL-6, corticosterone (CORT) and adrenocorticotropic hormone (ACTH) as well as increased neuronal apoptosis. These effects were indicative of radiation-induced bystander effects. Administration of HQD attenuated the pathological damage to brain and kidney tissues, and increased the numbers of WBC, neutrophils, lymphocyte and monocytes, as well as the expression of IL-2, CRH and PCNA. It also decreased the expression of IL-1 beta , IL-6, CORT and ACTH as well as neuronal apoptosis. HQD exhibits protective effects against 12C6+ radiation-induced bystander effects. The underlying mechanism may involve the promotion of the production of peripheral blood cells, inhibition of inflammatory factors and apoptosis, and regulation of endocrine hormones.