文献类型：期刊文献

英文题名：Astragalus Saponins and Liposome Constitute an Efficacious Adjuvant Formulation for Cancer Vaccines

作者：Zhang, Xiao-Ping[1];Li, Ying-Dong[1];Luo, Lu-Lu[2];Liu, Yong-Qi[1];Li, Yang[1];Guo, Chao[1];Li, Zhen-Dong[3];Xie, Xiao-Rong[1];Song, Hai-Xia[4];Yang, Li-Ping[5];Sun, Shao-Bo[1];An, Fang-Yu[1]

第一作者：张小平

通信作者：Li, YD[1]

机构：[1]Gansu Univ Chinese Med, Inst Integrated Tradit Chinese & Western Med, Dingxi East Rd 35, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou, Gansu, Peoples R China;[3]Lanzhou Univ, Dept Ultrasound, Hosp 2, Lanzhou, Gansu, Peoples R China;[4]Tumor Hosp Gansu Prov, Dept Radiotherapy, Lanzhou, Gansu, Peoples R China;[5]Lanzhou Univ, Dept Oncol, Hosp 1, Lanzhou, Gansu, Peoples R China

第一机构：甘肃中医药大学中西医结合学院

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Inst Integrated Tradit Chinese & Western Med, Dingxi East Rd 35, Lanzhou 730000, Gansu, Peoples R China.|[10735ed249c6606940a33]甘肃中医药大学中西医结合学院;[10735]甘肃中医药大学;

年份：2018

卷号：33

期号：1

起止页码：25

外文期刊名：CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS

收录：;Scopus(收录号：2-s2.0-85042480287);WOS:【SCI-EXPANDED(收录号:WOS:000425763200005)】；

基金：The work mainly performed in Gansu University of Chinese Medicine, Lanzhou, Gansu, China. This work was supported by the Natural Science Foundation of Gansu Science and Technology Department (Grant No. 1208RJZA221), National Natural Science Foundation of China (Grant No. 81360345), and Opening Foundation of Dunhuang Medicine and Transformation Key Laboratory of Ministry of Education (Grant No. DHYX17-07).

语种：英文

外文关键词：adjuvant formulation; Astragalus saponins; tumor vaccine

摘要：Cancer vaccines mostly aim to induce cytotoxic T lymphocytes (CTLs) against tumors. An appropriate adjuvant is of fundamental importance for inducing cellular immune response. Since the antigen in particulate form is substantially more immunogenic than soluble form antigen, it is beneficial to interact with antigen-presenting cells membrane to induce robust CD8(+) T cell activation following vaccination. Based on previous research, we designed an adjuvant formulation by combining Astragalus saponins, cholesterol, and liposome to incorporate antigen into a particulate delivery system, so as to enhance cellular immune response. Meanwhile, angiogenesis contributes to tumor growth and metastasis, and basic fibroblast growth factor (bFGF) is involved in tumor angiogenesis. Therefore, using lipo-saponins adjuvant formulation and a human recombinant bFGF antigen protein, we tried to induce bFGF-specific CTL response to inhibit tumor angiogenesis to achieve antitumor activity. After five immunizations, the lipo-saponins/bFGF complex elicited robust antibody response and markedly higher amount of interferon- in BALB/c mice, resulting in superior antitumor activities. Decreased microvessel density in CD31 immunohistochemistry and the lysis of vascular endothelial cells by the T lymphocytes from the immunized mice indicated that the immunity inhibited the angiogenesis of tumors and further led to the inhibition of tumors. Our data suggest that the approach to construct adjuvant formulation between liposome and Astragalus saponins appeared highly desirable, and that Astragalus saponins may be utilized as a valuable additive for enhancing the effectiveness of vaccines and stimulating an appropriate immune response that can benefit tumor therapy.