文献类型：期刊文献

英文题名：Gallic acid inhibits EMCV infection via targeting the interaction between TBK1 and IRF3 to promote IFN-β expression

作者：Zhang, Yan[1,2];Wen, Yanqiao[3,4];Xue, Weijiao[1];Zhang, Tengyu[1];Hou, Jixia[1];Chen, Xuewen[3];Feng, Ruofei[3];Tan, Chunxia[1,2]

第一作者：Zhang, Yan;张岩;张炎;张燕;张艳

通信作者：Tan, CX[1];Tan, CX[2];Feng, RF[3]

机构：[1]Gansu Univ Chinese Med, Lanzhou, Peoples R China;[2]Res Ctr Tradit Chinese Med, Lanzhou, Peoples R China;[3]Northwest Minzu Univ, Biomed Res Ctr, Key Lab Biotechnol & Bioengn State Ethn Affairs Co, Lanzhou, Peoples R China;[4]Northwest Minzu Univ, Sch Life Sci & Engn, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou, Peoples R China;[2]corresponding author), Res Ctr Tradit Chinese Med, Lanzhou, Peoples R China;[3]corresponding author), Northwest Minzu Univ, Biomed Res Ctr, Key Lab Biotechnol & Bioengn State Ethn Affairs Co, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

外文期刊名：MICROBIOLOGY SPECTRUM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001596060700001)】；

基金：This work was supported by Research Center of Traditional Chinese Medicine, Gansu Province (zyzx-2023-22).

语种：英文

外文关键词：gallic acid; EMCV; antiviral; IFN-beta

摘要：Encephalomyocarditis virus (EMCV) is an important zoonotic pathogen with global distribution, which has caused huge serious economic losses to the development of the livestock industry. Gallic acid (GA) is an active ingredient of traditional Chinese medicine with wide pharmacological and biological activities and is also a potential antiviral drug. However, its antiviral effect and mechanism of anti-EMCV are still unclear. In the present study, we found that therapeutic administration of GA could significantly reduce the viral load and viral titer of EMCV to inhibit EMCV replication in a dose-dependent manner. GA could also protect cells infected by EMCV and decrease the content of capsid protein VP1 of EMCV in HEK-293 cells. Additionally, the mechanistic investigations revealed that GA might exert antiviral effects by regulating the interaction between TBK1 and IRF3 to promote the IFN-beta expression. Meanwhile, GA could also alleviate EMCV-infected mice. These results indicate that GA may serve as a novel antiviral agent against EMCV infection.IMPORTANCEAs a zoonotic pathogen, encephalomyocarditis virus (EMCV) causes myocarditis, encephalitis, neurological disease, reproductive disorders, and diabetes in pigs, which seriously endangers the development of the swine industry worldwide. However, due to the lack of effective commercial vaccines, there is an urgent need to develop safe and effective drugs against EMCV. Gallic acid (GA) has wide pharmacological and biological activities. However, the antiviral effect and the mechanism of GA are currently unknown. Here, we demonstrated that GA had a significant anti-EMCV effect. Further research found that GA inhibited EMCV infection via targeting the interaction between TBK1 and IRF3 to promote the IFN-beta expression. These findings indicate that GA could be an effective anti-EMCV drug.