文献类型：期刊文献

中文题名：蛇葡萄素钠协同卡铂对肺癌Lewis细胞增殖的抑制作用

英文题名：Inhibitory Effects of Ampelopsin Sodium Combined with Carboplatin on the Proliferation of Lewis Cell of Lung Cancer

作者：缪苗苗[1,3];张艳霞[2,3];吴勇杰[3];覃红[3]

第一作者：缪苗苗

机构：[1]兰州市第二人民医院,兰州730046;[2]甘肃中医学院科研实验中心,兰州730000;[3]兰州大学基础医学院药理学研究所/甘肃省新药临床前研究重点实验室,兰州730000

第一机构：兰州市第二人民医院,兰州730046

年份：2015

卷号：26

期号：19

起止页码：2664

中文期刊名：中国药房

外文期刊名：China Pharmacy

收录：CSTPCD;;北大核心:【北大核心2014】；

语种：中文

中文关键词：蛇葡萄素钠;卡铂;肺癌Lewis细胞;半胱氨酸天冬氨酸蛋白水解酶3

外文关键词：Ampelopsin sodium; Carboplatin; Lewis cells of lung cancer; Caspase-3

摘要：目的：研究蛇葡萄素钠（AMP-Na）协同卡铂对肺癌Lewis细胞增殖的抑制作用。方法：以6.25、12.5、25、50、100μg/ml AMP-Na＋3.125、6.25、12.5、25、50μg/ml卡铂培养细胞4 h,测定细胞活力并计算抑制率与半数抑制浓度（IC50）。以12.5、25、50、100μg/ml AMP-Na＋12.5μg/ml卡铂培养细胞12 h,流式细胞仪检测细胞半胱氨酸天冬氨酸蛋白水解酶3（Caspase-3）表达。结果：系列质量浓度AMP-Na与系列质量浓度卡铂联用后对细胞生长有明显抑制作用,随质量浓度的升高,卡铂对Lewis细胞的IC50逐渐降低;AMP-Na质量浓度范围在6.25~50μg/ml时,与3.125~25μg/ml卡铂联用后,对Lewis细胞增殖的协同抑制作用最强。AMP-Na与卡铂联合培养细胞12 h后,细胞Caspase-3表达明显增强。结论：AMP-Na与卡铂合用对Lewis细胞增殖具有协同抑制效应,其机制可能与AMP-Na激活细胞Caspase-3,从而诱导细胞凋亡有关。
OBJECTIVE：To study the inhibitory effects of ampelopsin sodium（AMP-Na） combined with carboplatin on the proliferation of Lewis cell of lung cancer.METHODS：6.25,12.5,25,50 and 100 μg/ml AMP-Na and 3.125,6.25,12.5,25 and50 μg/ml carboplatin were used to culture the cells for 4 h,and the cell viability was determined and the inhibition rate and half inhibition concentration（IC50）were calculated.12.5,25,50 and 100 μg/ml AMP-Na and 12.5 μg/ml carboplatin were used to culture the cells for 12 h,and the flow cytometry was used to determine the expression of Caspase-3.RESULTS：AMP-Na with serial concentrations combined with carboplatin with serial concentrations had obvious inhibitory effects on the cell proliferation.With the increase of mass concentration,the IC50 of carboplatin on the Lewis cells was gradually decreased;when the AMP-Na of 6.25-50μg/ml was combined with carboplatin of 3.125-25 μg/ml,it showed the strongest inhibitory effects on the Lewis cell proliferation.When cells were cultured with AMP-Na and carboplatin for 24 h,the expression of Caspase-3 increased significantly.CONCLUSIONS：AMP-Na combined with carboplatin has synergistic inhibitory effect on the Lewis cell proliferation by a mechanism that may be related to the apoptosis induced by Caspase-3 activated by AMP-Na.