文献类型：期刊文献

中文题名：参芪抑瘤方药物血清对胃癌MKN-45细胞周期阻滞及抗侵袭转移的影响

英文题名：Effects of Shenqi Yiliu Decoction Medicated Serum on Blocking Cell Cycle and Inhibiting Invasion and Metastasis of Gastric Cancer Cell Lines MKN-45

作者：马春林[1];吴红彦[1,2,3];李海龙[1,2];陈杰[1];张宣[1]

第一作者：马春林

机构：[1]甘肃中医药大学;[2]甘肃省中医方药挖掘和创新转化重点实验室;[3]甘肃省中药新产品创制工程实验室

第一机构：甘肃中医药大学

年份：2016

卷号：23

期号：4

起止页码：64

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2015_2016】；

基金：甘肃省高等学校科研项目(2013A-088)

语种：中文

中文关键词：参芪抑瘤方;胃癌;MKN-45细胞;细胞周期;COX-2蛋白;PTEN蛋白;大鼠

外文关键词：Shenqi Yiliu Decoction;;gastric cancer;;MKN-45 cell;;cell cycle;;COX-2;;PTEN;;rats

摘要：目的 探讨不同浓度参芪抑瘤方药物血清对胃癌MKN-45 细胞周期阻滞及抗侵袭转移作用的相关机制.方法 60 只SPF 级Wistar 大鼠,随机分为对照组和参芪抑瘤方低、中、高剂量组,每组15 只.参芪抑瘤方低、中、高剂量组分别给予0.25、0.50、1.00 g 原药材/mL 药液灌胃,空白组给予等量饮用水灌胃,每日2 次,连续7 d,末次灌胃2 h 后取血,分离血清.MKN-45 细胞经不同浓度药物血清处理后,流式细胞术检测细胞周期,免疫组化检测COX-2、PTEN 蛋白表达.结果 药物血清干预后细胞G0～G1 期比例增加,S 期比例减少;药物血清可降低MKN-45 细胞COX-2 蛋白阳性表达率,升高PTEN 蛋白阳性表达率（P〈0.05）.结论 参芪抑瘤方药物血清阻滞细胞周期及抗侵袭转移作用可能与影响COX-2、PTEN 蛋白表达有关.
Objective To investigate the relevant mechanism of different concentrations of Shenqi YiliuDecoction medicated serum on blocking cell cycle and inhibiting invasion and metastasis of gastric cancer cell lines MKN-45. Methods Sixty SPF Wistar rats were randomized intoShenqi Yiliu Decoction low-, medium-, and high-dose groups and control group, with 15 rats in each group. Low-, medium- and high-dose groups were fed with different concentrations of TCM liquid from which containing crude medicine (0.25, 0.50, 1.00 g/mL) separately for gavage. Rats in the blank group were given the same amount of drinking water for gavage, twice a day, for 7 days.2 h after the last gavage, rat blood was taken and serum was separated. After MKN-45 cells were dealt with different concentrations of medicinal serum, FCM was used to detect cell cycle and immunohistochemistry technology was used to detect the expressions of COX-2 and PTEM proteins.Results FCM analysis showed that medicated serum could increase G0-G1 phase and shorten S phase of cells; medicated serum could reduce the positive expression rate of COX-2 of the MKN-45 cells and increase positive expression rate of PTEN proteins (P<0.05).ConclusionShenqi YiliuDecoction medicated serum can block the cell cycle and inhibit invasion and metastasis of MKN-45 cell lines, which may be related to the intervention in the expression levels of COX-2 and PTEN proteins.