文献类型：期刊文献

英文题名：Solid-waste-based keratin/chitosan hydrogel for controlling drug release in vitro

作者：Yin, Xiaochun[1];Du, Meixia[2];Sun, Zhongming[2];Zhu, Hai[2];Xu, Pei[1];Wang, Huiyao[1]

第一作者：Yin, Xiaochun

通信作者：Yin, XC[1];Wang, HY[1]

机构：[1]New Mexico State Univ, Dept Civil Engn, Las Cruces, NM 88003 USA;[2]Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou 730000, Gansu, Peoples R China

第一机构：New Mexico State Univ, Dept Civil Engn, Las Cruces, NM 88003 USA

通信机构：[1]corresponding author), New Mexico State Univ, Dept Civil Engn, Las Cruces, NM 88003 USA.

年份：2023

卷号：199

外文期刊名：EUROPEAN POLYMER JOURNAL

收录：;EI(收录号：20234014836807);Scopus(收录号：2-s2.0-85172993772);WOS:【SCI-EXPANDED(收录号:WOS:001087451400001)】；

语种：英文

外文关键词：Biomaterial; Keratin; Chitosan; Hydrogel; Drug delivery systems

摘要：The significant quantity of keratin-based solid waste presents a substantial challenge in proper disposal and requires effective management to reduce potential environmental risks. A promising strategy involves the conversion of keratin waste into valuable keratin-based hydrogels. However, these hydrogels typically exhibit insufficient mechanical properties. To address this limitation, this study aimed to develop hybrid hydrogels by integrating keratin with chitosan through alkaline freeze-thaw cycles. The resulting hydrogels, with various ratios, were characterized using scanning electron microscopy and Fourier transform infrared spectroscopy. The analysis revealed the hydrogels had a honeycomb-like structure with excellent mechanical attributes. Among the prepared hydrogels, hydrogel C with keratin/chitosan of 3/2 exhibited a compressive stress and strain of 2.15 MPa and 65.1 %, respectively. The drug loading capacities and controlled release behaviors of the hydrogels were extensively evaluated. Among the various hydrogels tested, hydrogel C demonstrated the most effective controlled release profiles for Rhodamine B (RB) and bovine serum albumin (BSA) at different pH and temperatures. At pH 7.4, hydrogel C achieved maximum cumulative releases of 81.7 % for RB at 50 degrees C and 31.2 % for BSA at 27 degrees C. The attainment of equilibrium release occurred after approximately 8 h for RB and 44 h for BSA at 37 degrees C. In addition, hydrogel C demonstrated exceptional loading properties and controlled release capabilities for cisplatin (CDDP, an anti-cancer drug) without inducing cytotoxic effects on normal and cancer cells. However, CDDP-loaded hydrogel C consistently exhibited cytotoxicity against cancer cells in vitro. These findings collectively underscore the promising potential of keratin/chitosan hybrid hydrogels as drug carriers.