文献类型：期刊文献

中文题名：针刺对单眼剥夺弱视大鼠视皮层17区神经元异常时空模式的干预研究

英文题名：Intervention of acupuncture on abnormal spatiotemporal pattern of visual cortex area 17 neuron in monocular deprivation amblyopia rats

作者：朱田田[1];马重兵[2];严兴科[1]

第一作者：朱田田

机构：[1]甘肃中医药大学针灸推拿学院,兰州730000;[2]兰州大学第一医院东岗院区中西医结合科

第一机构：甘肃中医药大学针灸推拿学院

年份：2017

卷号：37

期号：1

起止页码：61

中文期刊名：中国针灸

外文期刊名：Chinese Acupuncture & Moxibustion

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；PubMed;

基金：国家自然科学基金项目:81260560

语种：中文

中文关键词：单眼剥夺,敏感期;微电极技术;针刺干预;时空模式

外文关键词：monocular deprivation; sensitive period; microelectrode technique; acupuncture intervention; spatiotemporal

摘要：目的:初步揭示针刺对视皮层可塑性变化的电生理调节机制。方法:14d龄Wistar大鼠50只,随机分为空白组、模型组、敏感早期、敏感中期和敏感末期针刺组,每组10只。除空白组外,余组采用缝合上下眼睑的方法复制单眼剥夺弱视大鼠模型。造模后,空白组和模型组每天抓取,不予其他干预;各针刺组均选取双侧"睛明""攒竹""光明""风池",分别于模型复制后的第3d、12d、21d开始针刺干预,每次留针10min,每日针刺1次,共9d。干预结束后采用在体多通道神经信号技术(M-NEMEA)检测各组大鼠视皮层17区神经元放电波的幅值和功率谱密度。结果:与空白组相比,模型组大鼠视皮层17区放电神经元减少,波幅明显降低(P<0.05);敏感早期、中期针刺组大鼠放电神经元增多,波幅较模型组均显著升高(均P<0.05);敏感早期针刺组神经元放电波幅值高于敏感中期针刺组和敏感末期针刺组(均P<0.05),敏感中期针刺组神经元放电波幅值高于敏感末期针刺组(P<0.05)。在120s采集时域内,空白组功率谱密度主要集中在-105^-100dB,模型组功率谱密度区间较空白组增大,主要分布在-132^-124dB;各针刺组功率谱密度区间较模型组缩小,敏感早期针刺组功率谱密度主要分布在-115^-110dB,敏感中期针刺组主要分布于-120^-115dB,敏感末期针刺组主要分布于-129^-122dB。结论:单眼剥夺后大鼠视皮层17区神经元出现时空模式异常改变,而针刺对神经元时空模式改变有明显的调节作用,表明敏感期内视皮层神经元存在发育可塑性,早期治疗是取得疗效的关键。
Objective To preliminarily explore the electrophysiological mechanism of acupuncture on plasticity variability of visual cortex.Methods Fifty 14-day-old Wistar rats were randomly divided into a blank group,a model group,an early-stage acupuncture group,a middle-stage acupuncture group and a late-stage acupuncture group,10 rats in each one.Except the blank group,the rats in the remaining groups were treated with eyelid suture to establish the model of monocular deprivation amblyopia.After model establishment,the rats in the blank group and model group were grasped without any intervention everyday.The rats in the acupuncture groups were treated with acupuncture at bilateral＂Jingming＂（BL 1）,＂Cuanzhu＂（BL 2）,＂Fengchi＂（GB 20）and ＂Guangming＂（GB 37）on the 3rd,12 th and 21 st days into model establishment.The needles were retained for 10 min,once a day for totally 9days.After treatment,the M-NEMEA technology was applied to measure the discharge amplitude and power spectral density of visual cortex area 17 neuron of each group.Results Compared with the blank group,the number of discharged neuron of visual cortex area 17 was reduced,and the amplitude was significantly reduced in the model group（both P〈0.05）.Compared with the model group,the numbers of discharged neuron were increased,and the amplitudes were significantly increased in the early-stage acupuncture group and middle-stage acupuncture group（all P〈0.05）.The neuron discharge amplitude in the early-stage acupuncture group was higher than those in the middle-stage acupuncture group and late-stage acupuncture group（both P〈0.05）;the neuron discharge amplitude in the middle-stage acupuncture group was higher than that in the late-stage acupuncture group（P〈0.05）.In the 120-second time domain of acquisition,the distribution of power spectral density in the blank group was mainly between -105 dB and -100dB;compared with the blank group,the power spectral density in the model group was increased and distributed between -132 dB and -124dB;compared with the model group,the power spectral densities in the acupuncture groups was reduced and distributed between -115 dB and -110 dB in the early-stage acupuncture group,-120 dB and -115 dB in the middle-stage acupuncture group and -129 dB and -122 dB in the late-stage acupuncture group.Conclusion There are abnormal changes of spatiotemporal pattern in visual cortex area 17 of monocular deprivation rats,but acupuncture can regulate abnormal spatiotemporal pattern of neuron.Therefore,there is developmental plasticity of visual cortex neuron in the sensitive period,and early treatment is key to superior efficacy.