文献类型：期刊文献

英文题名：Oridonin Alleviates Visceral Hyperalgesia in a Rat Model of Postinflammatory Irritable Bowel Syndrome: Role of Colonic Enterochromaffin Cell and Serotonin Availability

作者：Zang, Kai-hong[1];Shao, Yun-yun[2,3];Zuo, Xiao[2,3];Rao, Zhi[2];Qin, Hong-yan[2]

第一作者：臧凯宏

通信作者：Zang, KH[1];Qin, HY[2]

机构：[1]Gansu Univ Tradit Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[2]Lanzhou Univ, Hosp 1, Dept Pharm, Lanzhou 730000, Peoples R China;[3]Lanzhou Univ, Sch Pharmaceut, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[2]corresponding author), Lanzhou Univ, Hosp 1, Dept Pharm, Lanzhou 730000, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2016

卷号：19

期号：6

起止页码：586

外文期刊名：JOURNAL OF MEDICINAL FOOD

收录：;Scopus(收录号：2-s2.0-84974602745);WOS:【SCI-EXPANDED(收录号:WOS:000377433000009)】；

基金：This work was supported by the National Science Foundation of China ( 81341145) and the Fundamental Research Funds for the Central Universities (lzujbky-2014-223).

语种：英文

外文关键词：colonic cytokine production; enterochromaffin cell; oridonin; postinflammatory irritable bowel syndrome; serotonin

摘要：The aim of this present study was to investigate the effect of oridonin on visceral hyperalgesia and colonic serotonin availability in a rat model of trinitrobenzenesulfonic acid-induced postinflammatory irritable bowel syndrome (PI-IBS). Rats were randomly divided into five groups: normal control, PI-IBS model, PI-IBS+low-dose oridonin (5 mg/kg), PI-IBS+median-dose oridonin (10 mg/kg), and PI-IBS+high-dose oridonin (20 mg/kg). Rats in control and model groups were orally administered with water by gavage, whereas rats in oridonin-treated groups were orally administered with different dosages of oridonin, and drugs were given for 14 consecutive days. Compared with the control group, the pain threshold pressure was significantly reduced in PI-IBS rats. The colonic enterochromaffin (EC) cell number, serotonin content, and the protein expression of tryptophan hydroxylase (TPH) were markedly increased and the protein expression of serotonin reuptake transporter was significantly decreased in PI-IBS rats. The spleen index in PI-IBS rats was decreased, and the levels of tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, interleukin (IL)-4, and IL-13 in the colon of PI-IBS rats were also markedly decreased. Oridonin treatment dose dependently increased pain threshold pressure, and markedly decreased colon EC cell numbers, TPH expression, and serotonin content in PI-IBS rats. Oridonin treatment also significantly increased the spleen index as well as the levels of TNF-alpha, IFN-gamma, IL-4, and IL-13 in the colon of PI-IBS rats. Results of this study demonstrate that the analgesic effect of oridonin in PI-IBS rats is associated with reduced colonic EC cell hyperplasia and 5-HT availability, the regulatory effect of oridonin on colonic cytokine production may be correlated with its effect on colonic EC cell number.